Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
Abstract
The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
- Inventors:
-
- Richland, WA
- Fall City, WA
- Pasco, WA
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 956997
- Patent Number(s):
- 7449569
- Application Number:
- 10/920,625
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- DE-AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States: N. p., 2008.
Web.
Dai, Ziyu, Lasure, Linda L, & Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States.
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Tue .
"Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus". United States. https://www.osti.gov/servlets/purl/956997.
@article{osti_956997,
title = {Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus},
author = {Dai, Ziyu and Lasure, Linda L and Magnuson, Jon K},
abstractNote = {The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {11}
}
Works referenced in this record:
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journal, August 1987
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journal, December 1987
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- The EMBO Journal, Vol. 6, Issue 13
Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi
journal, March 1989
- Roberts, I. N.; Oliver, R. P.; Punt, P. J.
- Current Genetics, Vol. 15, Issue 3