Methods for increasing the production of ethanol from microbial fermentation
Abstract
A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.
- Inventors:
-
- Fayetteville, AR
- Bethlehem, PA
- Issue Date:
- Research Org.:
- BIOENGINEERING RESOURCES, INC.
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 941118
- Patent Number(s):
- 7285402
- Application Number:
- 10/311,655
- Assignee:
- Emmaus Foundation, Inc. (Fayetteville, AK)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC04-94AL98770
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, and Clausen, Edgar C. Methods for increasing the production of ethanol from microbial fermentation. United States: N. p., 2007.
Web.
Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, & Clausen, Edgar C. Methods for increasing the production of ethanol from microbial fermentation. United States.
Gaddy, James L, Arora, Dinesh K, Ko, Ching-Whan, Phillips, John Randall, Basu, Rahul, Wikstrom, Carl V, and Clausen, Edgar C. Tue .
"Methods for increasing the production of ethanol from microbial fermentation". United States. https://www.osti.gov/servlets/purl/941118.
@article{osti_941118,
title = {Methods for increasing the production of ethanol from microbial fermentation},
author = {Gaddy, James L and Arora, Dinesh K and Ko, Ching-Whan and Phillips, John Randall and Basu, Rahul and Wikstrom, Carl V and Clausen, Edgar C},
abstractNote = {A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {10}
}
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