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Title: Recombinant constructs of Borrelia burgdorferi

Abstract

Novel chimeric nucleic acids, encoding chimeric Borrelia proteins comprising OspC or an antigenic fragment thereof and OspA or an antigenic fragment thereof, are disclosed. Chimeric proteins encoded by the nucleic acid sequences are also disclosed. The chimeric proteins are useful as vaccine immunogens against Lyme borreliosis, as well as for immunodiagnostic reagents.

Inventors:
 [1];  [2];  [3];  [4]
  1. Setauket, NY
  2. New York, NY
  3. East Setauket, NY
  4. Bellport, NY
Issue Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
919842
Patent Number(s):
7179448
Assignee:
Research Foundation of the State of New York (Stony Brook, NY); Brookhaven Sciences Associates, LLC (Upton, NY)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Dattwyler, Raymond J, Gomes-Solecki, Maria J. C., Luft, Benjamin J, and Dunn, John J. Recombinant constructs of Borrelia burgdorferi. United States: N. p., 2007. Web.
Dattwyler, Raymond J, Gomes-Solecki, Maria J. C., Luft, Benjamin J, & Dunn, John J. Recombinant constructs of Borrelia burgdorferi. United States.
Dattwyler, Raymond J, Gomes-Solecki, Maria J. C., Luft, Benjamin J, and Dunn, John J. Tue . "Recombinant constructs of Borrelia burgdorferi". United States. https://www.osti.gov/servlets/purl/919842.
@article{osti_919842,
title = {Recombinant constructs of Borrelia burgdorferi},
author = {Dattwyler, Raymond J and Gomes-Solecki, Maria J. C. and Luft, Benjamin J and Dunn, John J},
abstractNote = {Novel chimeric nucleic acids, encoding chimeric Borrelia proteins comprising OspC or an antigenic fragment thereof and OspA or an antigenic fragment thereof, are disclosed. Chimeric proteins encoded by the nucleic acid sequences are also disclosed. The chimeric proteins are useful as vaccine immunogens against Lyme borreliosis, as well as for immunodiagnostic reagents.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {2}
}

Works referenced in this record:

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journal, April 1997


Structural analysis of an outer surface protein from the Lyme disease spirochete, Borrelia burgdorferi, using circular dichroism and fluorescence spectroscopy
journal, March 1992


Expression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus ticks removed from humans
journal, October 1998


Mapping antibody-binding domains of the major outer surface membrane protein (OspA) of Borrelia burgdorferi.
journal, January 1991


Seroprotective groups among isolates of Borrelia burgdorferi.
journal, January 1993


Protection of mice against the Lyme disease agent by immunizing with recombinant OspA
journal, October 1990


Vaccination of hamsters against experimental infection with Borrelia burgdorferi
journal, December 1986

  • Johnson, Russell C.; Kodner, Carrie L.; Russell, Marie E.
  • Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, Vol. 263, Issue 1-2
  • https://doi.org/10.1016/S0176-6724(86)80101-8

Expression and secretion of outer surface protein (OSP-A) of Borrelia burgdorferi from Escherichia coli
journal, May 1993


Evaluation of Whole-Cell and OspC Enzyme-Linked Immunosorbent Assays for Discrimination of Early Lyme Borreliosis from OspA Vaccination
journal, January 2000


Molecular analysis of the outer surface protein A (OspA) of Borrelia burgdorferi for conserved and variable antibody binding domains
journal, September 1992


An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis.
journal, January 1993


Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA.
journal, January 1993


Selection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B.
journal, January 1995


Protective Activity of Antisera against Isolates of Borrelia burgdorferi from Various Geographical Origins
journal, January 1993


Therapeutic passive vaccination against chronic Lyme disease in mice
journal, November 1997


Induction of an outer surface protein on Borrelia burgdorferi during tick feeding.
journal, March 1995


Distribution and molecular analysis of Lyme disease spirochetes, Borrelia burgdorferi, isolated from ticks throughout California.
journal, January 1993


A mouse model for Borrelia burgdorferi infection: approach to a vaccine against Lyme disease
journal, January 1991


Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine.
journal, January 1996


Immunization against Lyme Disease — An Important First Step
journal, July 1998


Elimination of Borrelia burgdorferi from vector ticks feeding on OspA-immunized mice.
journal, June 1992


Recombinant Chimeric Borrelia Proteins for Diagnosis of Lyme Disease
journal, January 2000


Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection.
journal, January 1997


Vaccination against Lyme borreliosis
journal, January 1999


Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii.
journal, January 1993


Outer surface lipoproteins of Borrelia burgdorferi activate vascular endothelium in vitro.
journal, January 1996


Immunological and molecular variability of OspA and OspC. implications forBorrelia vaccine development
journal, March 1996


Diversity of OspA and OspC among cerebrospinal fluid isolates ofBorrelia burgdorferi sensu lato from patients with neuroborreliosis in Germany
journal, February 1996


Spirochetes: vaccines, animal models and diagnostics
journal, January 1992


Long-term protection of mice from Lyme disease by vaccination with OspA.
journal, January 1992


Protective immunity is induced by a Borrelia burgdorferi mutant that lacks OspA and OspB.
journal, January 1993


Lyme disease: Pathogenesis and vaccine development
journal, December 1999


Passive immunization of hamsters against experimental infection with the Lyme disease spirochete.
journal, January 1986


Recombinant Outer Surface Protein A from Borrelia burgdorferi Induces Antibodies Protective against Spirochetal Infection in Mice
journal, July 1991


Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.
journal, November 1993


Variability of osp genes and gene products among species of Lyme disease spirochetes.
journal, January 1993


The Lyme Disease Vaccine: Conception, Development, and Implementation
journal, April 2000


Recombination between genes encoding major outer surface proteins A and B of Borrelia burgdorferi
journal, October 1992


Active immunization of hamsters against experimental infection with Borrelia burgdorferi.
journal, January 1986


Evidence for an α-helical epitope on outer surface protein A from the Lyme disease spirochete, Borrelia burgdorferi: An application of steady-state and time-resolved fluorescence quenching techniques
journal, October 1993


Disarming Lyme Disease
journal, September 1994


Experimental Infection of the Hamster with Borrelia burgdorferi
journal, August 1988


Seroprotective Groups of Lyme Borreliosis Spirochetes from North America and Europe
journal, July 1994


Arthropod- and host-specific gene expression by Borrelia burgdorferi.
journal, February 1997


Active immunization with pC protein ofBorrelia burgdorferi protects gerbils againstB. burgdorferi infection
journal, November 1992