skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Integrated crystal mounting and alignment system for high-throughput biological crystallography

Abstract

A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [8];  [9];  [10]
  1. San Leandro, CA
  2. Richmond, CA
  3. Antioch, CA
  4. Moraga, CA
  5. Oakland, CA
  6. Berkeley, CA
  7. Lafayette, CA
  8. Walnut Creek, CA
  9. Chicago, IL
  10. La Jolla, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
915744
Patent Number(s):
7274769
Application Number:
11/064,357
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Nordmeyer, Robert A, Snell, Gyorgy P, Cornell, Earl W, Kolbe, William F, Yegian, Derek T, Earnest, Thomas N, Jaklevich, Joseph M, Cork, Carl W, Santarsiero, Bernard D, and Stevens, Raymond C. Integrated crystal mounting and alignment system for high-throughput biological crystallography. United States: N. p., 2007. Web.
Nordmeyer, Robert A, Snell, Gyorgy P, Cornell, Earl W, Kolbe, William F, Yegian, Derek T, Earnest, Thomas N, Jaklevich, Joseph M, Cork, Carl W, Santarsiero, Bernard D, & Stevens, Raymond C. Integrated crystal mounting and alignment system for high-throughput biological crystallography. United States.
Nordmeyer, Robert A, Snell, Gyorgy P, Cornell, Earl W, Kolbe, William F, Yegian, Derek T, Earnest, Thomas N, Jaklevich, Joseph M, Cork, Carl W, Santarsiero, Bernard D, and Stevens, Raymond C. Tue . "Integrated crystal mounting and alignment system for high-throughput biological crystallography". United States. https://www.osti.gov/servlets/purl/915744.
@article{osti_915744,
title = {Integrated crystal mounting and alignment system for high-throughput biological crystallography},
author = {Nordmeyer, Robert A and Snell, Gyorgy P and Cornell, Earl W and Kolbe, William F and Yegian, Derek T and Earnest, Thomas N and Jaklevich, Joseph M and Cork, Carl W and Santarsiero, Bernard D and Stevens, Raymond C},
abstractNote = {A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {9}
}

Patent:

Save / Share:

Works referenced in this record:

Macromolecular Cryocrystallography
journal, June 1997