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Title: Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

Abstract

The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

Inventors:
 [1]
  1. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
915234
Patent Number(s):
7271241
Application Number:
10/423,688
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Waldo, Geoffrey S. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby. United States: N. p., 2007. Web.
Waldo, Geoffrey S. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby. United States.
Waldo, Geoffrey S. Tue . "Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby". United States. https://www.osti.gov/servlets/purl/915234.
@article{osti_915234,
title = {Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby},
author = {Waldo, Geoffrey S},
abstractNote = {The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {9}
}

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