Title: Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

Abstract

The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

Inventors:

Waldo, Geoffrey S ^[1]

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+ Show Inventor Affiliations

1. Santa Fe, NM

Issue Date:

2007-09-18

Research Org.:

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

915234

Patent Number(s):

7271241

Application Number:

10/423,688

Assignee:

Los Alamos National Security, LLC (Los Alamos, NM)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/00 - Fusion polypeptide
C07K14/43595 - {from coelenteratae, e.g. medusae}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/582 - {with fluorescent label}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K16/00 - Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies {

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/533 - with fluorescent label

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2317/565 - Complementarity determining region [CDR]

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DOE Contract Number:  

W-7405-ENG-36

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES