skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Enzymatic cascade bioreactor

Abstract

Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

Inventors:
 [1];  [2];  [3];  [4]
  1. San Francisco, CA
  2. Livermore, CA
  3. Albuquerque, NM
  4. Woodinville, WA
Issue Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
914637
Patent Number(s):
7264962
Application Number:
11/080,901
Assignee:
Sandia Corporation (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Enzymatic cascade bioreactor. United States: N. p., 2007. Web.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, & Walker, Andrew. Enzymatic cascade bioreactor. United States.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Tue . "Enzymatic cascade bioreactor". United States. https://www.osti.gov/servlets/purl/914637.
@article{osti_914637,
title = {Enzymatic cascade bioreactor},
author = {Simmons, Blake A and Volponi, Joanne V and Ingersoll, David and Walker, Andrew},
abstractNote = {Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {9}
}

Patent:

Save / Share:

Works referenced in this record:

Trienzymatic biosensor for the determination of inorganic phosphate
journal, September 2001


Enzymatic Conversion of Sucrose to Hydrogen
journal, December 1998


A Three-Cascaded-Enzymes Biosensor to Determine Lactose Concentration in Raw Milk
journal, September 2000


A Miniature Biofuel Cell
journal, September 2001


A Miniature Biofuel Cell Operating in A Physiological Buffer
journal, November 2002


Miniature biofuel cells
journal, January 2004


Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents
journal, June 2002


A Miniature Membrane-less Biofuel Cell Operating under Physiological Conditions at 0.5 V
journal, January 2003