Enzymatic cascade bioreactor
Abstract
Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.
- Inventors:
-
- San Francisco, CA
- Livermore, CA
- Albuquerque, NM
- Woodinville, WA
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 914637
- Patent Number(s):
- 7264962
- Application Number:
- 11/080,901
- Assignee:
- Sandia Corporation (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Enzymatic cascade bioreactor. United States: N. p., 2007.
Web.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, & Walker, Andrew. Enzymatic cascade bioreactor. United States.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Tue .
"Enzymatic cascade bioreactor". United States. https://www.osti.gov/servlets/purl/914637.
@article{osti_914637,
title = {Enzymatic cascade bioreactor},
author = {Simmons, Blake A and Volponi, Joanne V and Ingersoll, David and Walker, Andrew},
abstractNote = {Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {9}
}
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Works referenced in this record:
Trienzymatic biosensor for the determination of inorganic phosphate
journal, September 2001
- Mousty, Christine; Cosnier, Serge; Shan, Dan
- Analytica Chimica Acta, Vol. 443, Issue 1
Enzymatic Conversion of Sucrose to Hydrogen
journal, December 1998
- Woodward, J.; Orr, M.
- Biotechnology Progress, Vol. 14, Issue 6
A Three-Cascaded-Enzymes Biosensor to Determine Lactose Concentration in Raw Milk
journal, September 2000
- Eshkenazi, I.; Maltz, E.; Zion, B.
- Journal of Dairy Science, Vol. 83, Issue 9
A Miniature Biofuel Cell
journal, September 2001
- Chen, Ting; Barton, Scott Calabrese; Binyamin, Gary
- Journal of the American Chemical Society, Vol. 123, Issue 35
A Miniature Biofuel Cell Operating in A Physiological Buffer
journal, November 2002
- Mano, Nicolas; Mao, Fei; Heller, Adam
- Journal of the American Chemical Society, Vol. 124, Issue 44
Rapid determination of glucose and sucrose by an amperometric glucose-sensing electrode combined with an invertase/mutarotase-attached measuring cell
journal, November 1997
- Mizutani, Fumio; Yabuki, Soichi
- Biosensors and Bioelectronics, Vol. 12, Issue 9-10
Miniature biofuel cells
journal, January 2004
- Heller, Adam
- Physical Chemistry Chemical Physics, Vol. 6, Issue 2
Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents
journal, June 2002
- Gouda, M. D.; Kumar, M. A.; Thakur, M. S.
- Biosensors and Bioelectronics, Vol. 17, Issue 6-7
A Miniature Membrane-less Biofuel Cell Operating under Physiological Conditions at 0.5 V
journal, January 2003
- Kim, Hyug-Han; Mano, Nicolas; Zhang, Yongchao
- Journal of The Electrochemical Society, Vol. 150, Issue 2