Method and apparatus for detecting chemical binding
Abstract
The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 913240
- Patent Number(s):
- 7241381
- Application Number:
- 10/986,519
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Warner, Benjamin P, Havrilla, George J, Miller, Thomasin C, and Wells, Cyndi A. Method and apparatus for detecting chemical binding. United States: N. p., 2007.
Web.
Warner, Benjamin P, Havrilla, George J, Miller, Thomasin C, & Wells, Cyndi A. Method and apparatus for detecting chemical binding. United States.
Warner, Benjamin P, Havrilla, George J, Miller, Thomasin C, and Wells, Cyndi A. Tue .
"Method and apparatus for detecting chemical binding". United States. https://www.osti.gov/servlets/purl/913240.
@article{osti_913240,
title = {Method and apparatus for detecting chemical binding},
author = {Warner, Benjamin P and Havrilla, George J and Miller, Thomasin C and Wells, Cyndi A},
abstractNote = {The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 10 00:00:00 EDT 2007},
month = {Tue Jul 10 00:00:00 EDT 2007}
}
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