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Title: Destabilized bioluminescent proteins

Abstract

Purified nucleic acids, vectors and cells containing a gene cassette encoding at least one modified bioluminescent protein, wherein the modification includes the addition of a peptide sequence. The duration of bioluminescence emitted by the modified bioluminescent protein is shorter than the duration of bioluminescence emitted by an unmodified form of the bioluminescent protein.

Inventors:
 [1];  [2];  [3]
  1. Knoxville, TN
  2. New Delhi, IN
  3. Blaine, TN
Issue Date:
Research Org.:
University of Tennessee Research Foundation (Knoxville, TN)
Sponsoring Org.:
USDOE
OSTI Identifier:
913093
Patent Number(s):
7250284
Application Number:
10/827,133
Assignee:
University of Tennessee Research Foundation (Knoxville, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Allen, Michael S, Rakesh, Gupta, and Gary, Sayler S. Destabilized bioluminescent proteins. United States: N. p., 2007. Web.
Allen, Michael S, Rakesh, Gupta, & Gary, Sayler S. Destabilized bioluminescent proteins. United States.
Allen, Michael S, Rakesh, Gupta, and Gary, Sayler S. Tue . "Destabilized bioluminescent proteins". United States. https://www.osti.gov/servlets/purl/913093.
@article{osti_913093,
title = {Destabilized bioluminescent proteins},
author = {Allen, Michael S and Rakesh, Gupta and Gary, Sayler S},
abstractNote = {Purified nucleic acids, vectors and cells containing a gene cassette encoding at least one modified bioluminescent protein, wherein the modification includes the addition of a peptide sequence. The duration of bioluminescence emitted by the modified bioluminescent protein is shorter than the duration of bioluminescence emitted by an unmodified form of the bioluminescent protein.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {7}
}

Works referenced in this record:

Development of a Destabilized Firefly Luciferase Enzyme for Measurement of Gene Expression
journal, September 2000


Transferable Domain in the G1 Cyclin Cln2 Sufficient To Switch Degradation of Sic1 from the E3 Ubiquitin Ligase SCFCdc4 to SCFGrr1
journal, July 2002


Reporter Genes lucFF, luxCDABE, gfp, and dsred Have Different Characteristics in Whole-Cell Bacterial Sensors
journal, February 2002


tmRDB (tmRNA database)
journal, January 2001


Expression andin vivo determination of firefly luciferase as gene reporter inSaccharomyces cerevisiae
journal, October 1994


Control of Luminescence Decay and Flavin Binding by the LuxA Carboxyl-Terminal Regions in Chimeric Bacterial Luciferases
journal, October 1999


New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria
journal, June 1998


Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter
journal, December 1998