Dual fiber microprobe for mapping elemental distributions in biological cells

Martin, Rodger C; Martin, Madhavi Z

Title: Dual fiber microprobe for mapping elemental distributions in biological cells

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Abstract

Laser-induced breakdown spectroscopy (LIBS) is applied on a microscale for in situ elemental analysis and spatial mapping in biological cells. A high power laser beam is focused onto a cell surface using a dual branching optical fiber probe for optical excitation of the cell constituents. Dual spectrometers and ICCD detectors capture the emission spectra from the excited cell(s). Repeated probing or repositioning of the laser beam with respect to the cell can provide 2-D or 3-D mapping of the cell.

Inventors:

Martin, Rodger C ^[1]; Martin, Madhavi Z ^[1]

Powell, TN

Issue Date:: 2007-07-31

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 913092

Patent Number(s):: 7251022

Application Number:: 11/240,264

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G01J3/36 - Investigating two or more bands of a spectrum by separate detectors

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N21/6486 - {Measuring fluorescence of biological material, e.g. DNA, RNA, cells
G01N21/718 - {Laser microanalysis, i.e. with formation of sample plasma}

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DOE Contract Number:: AC05-00OR22725

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Martin, Rodger C, and Martin, Madhavi Z. Dual fiber microprobe for mapping elemental distributions in biological cells.  United States: N. p., 2007. 
        Web.

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                    Martin, Rodger C, & Martin, Madhavi Z. Dual fiber microprobe for mapping elemental distributions in biological cells.  United States.

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                    Martin, Rodger C, and Martin, Madhavi Z. Tue .  
        "Dual fiber microprobe for mapping elemental distributions in biological cells".  United States.  https://www.osti.gov/servlets/purl/913092.

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@article{osti_913092,

  title        = {Dual fiber microprobe for mapping elemental distributions in biological cells},

  author       = {Martin, Rodger C and Martin, Madhavi Z},

  abstractNote = {Laser-induced breakdown spectroscopy (LIBS) is applied on a microscale for in situ elemental analysis and spatial mapping in biological cells. A high power laser beam is focused onto a cell surface using a dual branching optical fiber probe for optical excitation of the cell constituents. Dual spectrometers and ICCD detectors capture the emission spectra from the excited cell(s). Repeated probing or repositioning of the laser beam with respect to the cell can provide 2-D or 3-D mapping of the cell.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2007},

  month        = {7}

}

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