Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
Abstract
A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.
- Inventors:
-
- Pinole, CA
- (Albany, CA)
- Oakland, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 913000
- Patent Number(s):
- 6699723
- Application Number:
- 09/865,130
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States: N. p., 2004.
Web.
Weiss, Shimon, Bruchez, Jr., Marcel, & Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States.
Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Tue .
"Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes". United States. https://www.osti.gov/servlets/purl/913000.
@article{osti_913000,
title = {Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes},
author = {Weiss, Shimon and Bruchez, Jr., Marcel and Alivisatos, Paul},
abstractNote = {A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {3}
}
Works referenced in this record:
Characterization of quantum-confined CdS nanocrystallites stabilized by deoxyribonucleic acid (DNA)
journal, April 1992
- Coffer, J. L.; Bigham, S. R.; Pinizzotto, R. F.
- Nanotechnology, Vol. 3, Issue 2
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998
- Chan, Warren C. W.; Nie, Shuming
- Science, Vol. 281, Issue 5385, p. 2016-2018
Quantum Dots as Inorganic DNA-Binding Proteins
journal, January 1996
- Murphy, Catherine J.; Brauns, Eric B.; Gearheart, Latha
- MRS Proceedings, Vol. 452
Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998
- Bruchez Jr., Marcel; Moronne, Mario Moronne; Gin, Peter Gin
- Science, Vol. 281, Issue 5385, p. 2013-2016
MICROSCOPY:Semiconductor Beacons Light Up Cell Structures
journal, September 1998
- Service, R. F.
- Science, Vol. 281, Issue 5385
Stabilization of CdS semiconductor nanoparticles against photodegradation by a silica coating procedure
journal, April 1998
- Correa-Duarte, Miguel A.; Giersig, Michael; Liz-Marzán, Luis M.
- Chemical Physics Letters, Vol. 286, Issue 5-6, p. 497-501
Multicolor Spectral Karyotyping of Human Chromosomes
journal, July 1996
- Schrock, E.; du Manoir, S.; Veldman, T.
- Science, Vol. 273, Issue 5274
Scintillation proximity assay: a versatile high-throughput screening technology
journal, July 1996
- Cook, Neil D.
- Drug Discovery Today, Vol. 1, Issue 7, p. 287-294
Organization of 'nanocrystal molecules' using DNA
journal, August 1996
- Alivisatos, A. Paul; Johnsson, Kai P.; Peng, Xiaogang
- Nature, Vol. 382, Issue 6592
Epitaxial Growth of Highly Luminescent CdSe/CdS Core/Shell Nanocrystals with Photostability and Electronic Accessibility
journal, July 1997
- Peng, Xiaogang; Schlamp, Michael C.; Kadavanich, Andreas V.
- Journal of the American Chemical Society, Vol. 119, Issue 30, p. 7019-7029
Synthesis of Nanosized Gold−Silica Core−Shell Particles
journal, January 1996
- Liz-Marzán, Luis M.; Giersig, Michael; Mulvaney, Paul
- Langmuir, Vol. 12, Issue 18
Semiconductor Clusters, Nanocrystals, and Quantum Dots
journal, February 1996
- Alivisatos, A. P.
- Science, Vol. 271, Issue 5251, p. 933-937
Synthesis and Isolatin of a Homodimer of Cadmium Selenide Nanocrystals
journal, February 1997
- Peng, Xiaogang; Wilson, Troy E.; Alivisatos, A. Paul
- Angewandte Chemie International Edition in English, Vol. 36, Issue 12, p. 145-147
Protein-Sized Quantum Dot Luminescence Can Distinguish between "Straight", "Bent", and "Kinked" Oligonucleotides
journal, September 1995
- Mahtab, Rahina; Rogers, Jessica P.; Murphy, Catherine J.
- Journal of the American Chemical Society, Vol. 117, Issue 35
Perspectives on the Physical Chemistry of Semiconductor Nanocrystals
journal, January 1996
- Alivisatos, A. P.
- The Journal of Physical Chemistry, Vol. 100, Issue 31
(CdSe)ZnS Core−Shell Quantum Dots: Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites
journal, November 1997
- Dabbousi, B. O.; Rodriguez-Viejo, J.; Mikulec, F. V.
- The Journal of Physical Chemistry B, Vol. 101, Issue 46, p. 9463-9475
QUANTUM DOTS MEET BIOMOLECULES: Method uses brightly fluorescing semiconductor nanocrystals as labels
journal, September 1998
- Jacoby, Mitch
- Chemical & Engineering News, Vol. 76, Issue 39
Preparation and microscopic visualization of multicolor luminescent immunophosphors
journal, January 1992
- Beverloo, H. B.; van Schadewijk, A.; Bonnet, J.
- Cytometry, Vol. 13, Issue 6
Electronic Energy Transfer in CdSe Quantum Dot Solids
journal, February 1996
- Kagan, C. R.; Murray, C. B.; Nirmal, M.
- Physical Review Letters, Vol. 76, Issue 9
Preferential Adsorption of a “Kinked” DNA to a Neutral Curved Surface: Comparisons to and Implications for Nonspecific DNA−Protein Interactions
journal, January 1996
- Mahtab, Rahina; Rogers, Jessica P.; Singleton, Chainey P.
- Journal of the American Chemical Society, Vol. 118, Issue 30