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Title: Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes

Abstract

A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

Inventors:
 [1];  [2];  [3]
  1. Pinole, CA
  2. (Albany, CA)
  3. Oakland, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
913000
Patent Number(s):
6699723
Application Number:
09/865,130
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States: N. p., 2004. Web.
Weiss, Shimon, Bruchez, Jr., Marcel, & Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States.
Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Tue . "Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes". United States. https://www.osti.gov/servlets/purl/913000.
@article{osti_913000,
title = {Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes},
author = {Weiss, Shimon and Bruchez, Jr., Marcel and Alivisatos, Paul},
abstractNote = {A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {3}
}

Works referenced in this record:

Characterization of quantum-confined CdS nanocrystallites stabilized by deoxyribonucleic acid (DNA)
journal, April 1992


Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998


Quantum Dots as Inorganic DNA-Binding Proteins
journal, January 1996


Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998


MICROSCOPY:Semiconductor Beacons Light Up Cell Structures
journal, September 1998


Stabilization of CdS semiconductor nanoparticles against photodegradation by a silica coating procedure
journal, April 1998


Multicolor Spectral Karyotyping of Human Chromosomes
journal, July 1996


Scintillation proximity assay: a versatile high-throughput screening technology
journal, July 1996


Organization of 'nanocrystal molecules' using DNA
journal, August 1996


Epitaxial Growth of Highly Luminescent CdSe/CdS Core/Shell Nanocrystals with Photostability and Electronic Accessibility
journal, July 1997

  • Peng, Xiaogang; Schlamp, Michael C.; Kadavanich, Andreas V.
  • Journal of the American Chemical Society, Vol. 119, Issue 30, p. 7019-7029
  • https://doi.org/10.1021/ja970754m

Synthesis of Nanosized Gold−Silica Core−Shell Particles
journal, January 1996


Semiconductor Clusters, Nanocrystals, and Quantum Dots
journal, February 1996


Synthesis and Isolatin of a Homodimer of Cadmium Selenide Nanocrystals
journal, February 1997


Protein-Sized Quantum Dot Luminescence Can Distinguish between "Straight", "Bent", and "Kinked" Oligonucleotides
journal, September 1995


Perspectives on the Physical Chemistry of Semiconductor Nanocrystals
journal, January 1996


(CdSe)ZnS Core−Shell Quantum Dots: Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites
journal, November 1997


Preparation and microscopic visualization of multicolor luminescent immunophosphors
journal, January 1992


Electronic Energy Transfer in CdSe Quantum Dot Solids
journal, February 1996


Preferential Adsorption of a “Kinked” DNA to a Neutral Curved Surface:  Comparisons to and Implications for Nonspecific DNA−Protein Interactions
journal, January 1996