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Title: Quenching methods for background reduction in luminescence-based probe-target binding assays

Abstract

Background luminescence is reduced from a solution containing unbound luminescent probes, each having a first molecule that attaches to a target molecule and having an attached luminescent moiety, and luminescent probe/target adducts. Quenching capture reagent molecules are formed that are capable of forming an adduct with the unbound luminescent probes and having an attached quencher material effective to quench luminescence of the luminescent moiety. The quencher material of the capture reagent molecules is added to a solution of the luminescent probe/target adducts and binds in a proximity to the luminescent moiety of the unbound luminescent probes to quench luminescence from the luminescent moiety when the luminescent moiety is exposed to exciting illumination. The quencher capture reagent does not bind to probe molecules that are bound to target molecules and the probe/target adduct emission is not quenched.

Inventors:
 [1];  [1];  [1];  [2]
  1. Los Alamos, NM
  2. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
908830
Patent Number(s):
7202036
Application Number:
10/460,451
Assignee:
Los Alamos National Security, LLC (Los Alamos, MN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Cai, Hong, Goodwin, Peter M, Keller, Richard A, and Nolan, Rhiannon L. Quenching methods for background reduction in luminescence-based probe-target binding assays. United States: N. p., 2007. Web.
Cai, Hong, Goodwin, Peter M, Keller, Richard A, & Nolan, Rhiannon L. Quenching methods for background reduction in luminescence-based probe-target binding assays. United States.
Cai, Hong, Goodwin, Peter M, Keller, Richard A, and Nolan, Rhiannon L. Tue . "Quenching methods for background reduction in luminescence-based probe-target binding assays". United States. https://www.osti.gov/servlets/purl/908830.
@article{osti_908830,
title = {Quenching methods for background reduction in luminescence-based probe-target binding assays},
author = {Cai, Hong and Goodwin, Peter M and Keller, Richard A and Nolan, Rhiannon L},
abstractNote = {Background luminescence is reduced from a solution containing unbound luminescent probes, each having a first molecule that attaches to a target molecule and having an attached luminescent moiety, and luminescent probe/target adducts. Quenching capture reagent molecules are formed that are capable of forming an adduct with the unbound luminescent probes and having an attached quencher material effective to quench luminescence of the luminescent moiety. The quencher material of the capture reagent molecules is added to a solution of the luminescent probe/target adducts and binds in a proximity to the luminescent moiety of the unbound luminescent probes to quench luminescence from the luminescent moiety when the luminescent moiety is exposed to exciting illumination. The quencher capture reagent does not bind to probe molecules that are bound to target molecules and the probe/target adduct emission is not quenched.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {4}
}

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Works referenced in this record:

Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer.
journal, December 1988


Self-Reporting PNA/DNA Primers for PCR Analysis
journal, April 2001