Single particle electrochemical sensors and methods of utilization
Abstract
The present invention discloses an electrochemical device for detecting single particles, and methods for using such a device to achieve high sensitivity for detecting particles such as bacteria, viruses, aggregates, immuno-complexes, molecules, or ionic species. The device provides for affinity-based electrochemical detection of particles with single-particle sensitivity. The disclosed device and methods are based on microelectrodes with surface-attached, affinity ligands (e.g., antibodies, combinatorial peptides, glycolipids) that bind selectively to some target particle species. The electrodes electrolyze chemical species present in the particle-containing solution, and particle interaction with a sensor element modulates its electrolytic activity. The devices may be used individually, employed as sensors, used in arrays for a single specific type of particle or for a range of particle types, or configured into arrays of sensors having both these attributes.
- Inventors:
-
- Oakland, CA
- Berkeley, CA
- Albuquerque, NM
- Los Gatos, CA
- Lafayette, CA
- Placitas, NM
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 908530
- Patent Number(s):
- 7022287
- Application Number:
- 10/141,806
- Assignee:
- Sandia National Laboratories (Livermore, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Schoeniger, Joseph, Flounders, Albert W, Hughes, Robert C, Ricco, Antonio J, Wally, Karl, Kravitz, Stanley H, and Janek, Richard P. Single particle electrochemical sensors and methods of utilization. United States: N. p., 2006.
Web.
Schoeniger, Joseph, Flounders, Albert W, Hughes, Robert C, Ricco, Antonio J, Wally, Karl, Kravitz, Stanley H, & Janek, Richard P. Single particle electrochemical sensors and methods of utilization. United States.
Schoeniger, Joseph, Flounders, Albert W, Hughes, Robert C, Ricco, Antonio J, Wally, Karl, Kravitz, Stanley H, and Janek, Richard P. Tue .
"Single particle electrochemical sensors and methods of utilization". United States. https://www.osti.gov/servlets/purl/908530.
@article{osti_908530,
title = {Single particle electrochemical sensors and methods of utilization},
author = {Schoeniger, Joseph and Flounders, Albert W and Hughes, Robert C and Ricco, Antonio J and Wally, Karl and Kravitz, Stanley H and Janek, Richard P},
abstractNote = {The present invention discloses an electrochemical device for detecting single particles, and methods for using such a device to achieve high sensitivity for detecting particles such as bacteria, viruses, aggregates, immuno-complexes, molecules, or ionic species. The device provides for affinity-based electrochemical detection of particles with single-particle sensitivity. The disclosed device and methods are based on microelectrodes with surface-attached, affinity ligands (e.g., antibodies, combinatorial peptides, glycolipids) that bind selectively to some target particle species. The electrodes electrolyze chemical species present in the particle-containing solution, and particle interaction with a sensor element modulates its electrolytic activity. The devices may be used individually, employed as sensors, used in arrays for a single specific type of particle or for a range of particle types, or configured into arrays of sensors having both these attributes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {4}
}