High-throughput, dual probe biological assays based on single molecule detection
Abstract
A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.
- Inventors:
-
- Brentwood, CA
- Livermore, CA
- Oakland, CA
- San Leandro, CA
- Pleasanton, CA
- (Patterson, CA)
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 908345
- Patent Number(s):
- 7076092
- Application Number:
- 10/170,876
- Assignee:
- The United States of America as represented by the United States Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Hollars, Christopher W, Huser, Thomas R, Lane, Stephen M, Balhorn, Rodney L, Bakajin, Olgica, Darrow, Christopher, and Satcher, Jr., Joe H. High-throughput, dual probe biological assays based on single molecule detection. United States: N. p., 2006.
Web.
Hollars, Christopher W, Huser, Thomas R, Lane, Stephen M, Balhorn, Rodney L, Bakajin, Olgica, Darrow, Christopher, & Satcher, Jr., Joe H. High-throughput, dual probe biological assays based on single molecule detection. United States.
Hollars, Christopher W, Huser, Thomas R, Lane, Stephen M, Balhorn, Rodney L, Bakajin, Olgica, Darrow, Christopher, and Satcher, Jr., Joe H. Tue .
"High-throughput, dual probe biological assays based on single molecule detection". United States. https://www.osti.gov/servlets/purl/908345.
@article{osti_908345,
title = {High-throughput, dual probe biological assays based on single molecule detection},
author = {Hollars, Christopher W and Huser, Thomas R and Lane, Stephen M and Balhorn, Rodney L and Bakajin, Olgica and Darrow, Christopher and Satcher, Jr., Joe H.},
abstractNote = {A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {7}
}
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