Flow cytometric detection method for DNA samples
Abstract
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.
- Inventors:
-
- Livermore, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 908290
- Patent Number(s):
- 7083951
- Application Number:
- W-7405-ENG-48
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Nasarabadi, Shanavaz, Langlois, Richard G, and Venkateswaran, Kodumudi S. Flow cytometric detection method for DNA samples. United States: N. p., 2006.
Web.
Nasarabadi, Shanavaz, Langlois, Richard G, & Venkateswaran, Kodumudi S. Flow cytometric detection method for DNA samples. United States.
Nasarabadi, Shanavaz, Langlois, Richard G, and Venkateswaran, Kodumudi S. Tue .
"Flow cytometric detection method for DNA samples". United States. https://www.osti.gov/servlets/purl/908290.
@article{osti_908290,
title = {Flow cytometric detection method for DNA samples},
author = {Nasarabadi, Shanavaz and Langlois, Richard G and Venkateswaran, Kodumudi S},
abstractNote = {Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {8}
}
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Works referenced in this record:
A Reusable Flow-Through Polymerase Chain Reaction Instrument for the Continuous Monitoring of Infectious Biological Agents
journal, July 2003
- Belgrader, Phillip; Elkin, Christopher J.; Brown, Steven B.
- Analytical Chemistry, Vol. 75, Issue 14
Flow Cytometry-Based Minisequencing: A New Platform for High-Throughput Single-Nucleotide Polymorphism Scoring
journal, June 2000
- Cai, Hong; White, P. Scott; Torney, David
- Genomics, Vol. 66, Issue 2
A handheld real time thermal cycler for bacterial pathogen detection
journal, August 2003
- Higgins, James A.; Nasarabadi, Shanavaz; Karns, Jeffrey S.
- Biosensors and Bioelectronics, Vol. 18, Issue 9
Fluorescent microsphere-based readout technology for multiplexed human single nucleotide polymorphism analysis and bacterial identification
journal, January 2001
- Ye, Fei; Li, May-Sung; Taylor, J. David
- Human Mutation, Vol. 17, Issue 4
Advanced multiplexed analysis with the FlowMetrixTM system
journal, September 1997
- Fulton, R. Jerrold; McDade, Ralph L.; Smith, Perry L.
- Clinical Chemistry, Vol. 43, Issue 9
Flow Cytometric Platform for High-Throughput Single Nucleotide Polymorphism Analysis
journal, March 2001
- Taylor, J. D.; Briley, D.; Nguyen, Q.
- BioTechniques, Vol. 30, Issue 3
A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
journal, April 2005
- Wilson, Wendy J.; Erler, Anne M.; Nasarabadi, Shanavaz L.
- Molecular and Cellular Probes, Vol. 19, Issue 2, p. 137-144
A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry
journal, October 2000
- Spiro, A.; Lowe, M.; Brown, D.
- Applied and Environmental Microbiology, Vol. 66, Issue 10
Autonomous Detection of Aerosolized Biological Agents by Multiplexed Immunoassay with Polymerase Chain Reaction Confirmation
journal, January 2005
- Hindson, Benjamin J.; McBride, Mary T.; Makarewicz, Anthony J.
- Analytical Chemistry, Vol. 77, Issue 1