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Title: Derivatized versions of ligase enzymes for constructing DNA sequences

Abstract

A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.

Inventors:
 [1];  [2];  [3];  [4];  [5]
  1. (Danville, CA)
  2. Tracy, CA
  3. Novi, MN
  4. Livermore, CA
  5. Oakland, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
908277
Patent Number(s):
7090979
Application Number:
10/718,856
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mariella, Jr., Raymond P., Christian, Allen T, Tucker, James D, Dzenitis, John M, and Papavasiliou, Alexandros P. Derivatized versions of ligase enzymes for constructing DNA sequences. United States: N. p., 2006. Web.
Mariella, Jr., Raymond P., Christian, Allen T, Tucker, James D, Dzenitis, John M, & Papavasiliou, Alexandros P. Derivatized versions of ligase enzymes for constructing DNA sequences. United States.
Mariella, Jr., Raymond P., Christian, Allen T, Tucker, James D, Dzenitis, John M, and Papavasiliou, Alexandros P. Tue . "Derivatized versions of ligase enzymes for constructing DNA sequences". United States. https://www.osti.gov/servlets/purl/908277.
@article{osti_908277,
title = {Derivatized versions of ligase enzymes for constructing DNA sequences},
author = {Mariella, Jr., Raymond P. and Christian, Allen T and Tucker, James D and Dzenitis, John M and Papavasiliou, Alexandros P},
abstractNote = {A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {8}
}

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Works referenced in this record:

Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase
journal, November 1970


Total synthesis of a gene
journal, February 1979


Total synthesis of a gene
journal, February 1979