Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms
Abstract
A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 894277
- Patent Number(s):
- 6989252
- Application Number:
- TRN: US200701%%293
- Assignee:
- NREL
- DOE Contract Number:
- AC36-99-GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 08 HYDROGEN; 59 BASIC BIOLOGICAL SCIENCES; CHLAMYDOMONAS; HYDROGEN; HYDROGEN PRODUCTION; IRON; MANGANESE; MICROORGANISMS; NUTRIENTS; OXYGEN; PRODUCTION; RESPIRATION; SULFUR; HYDROGENASE; OXYGENIC PHOTOSYNTHETIC ORGANISMS; ENDOGENOUS SUBSTRATE; PHOTOBIOLOGICAL HYDROGEN GAS PRODUCTION; Basic Sciences; Photoconversion; Hydrogen
Citation Formats
Melis, A, Zhang, L, Benemann, J R, Forestier, M, Ghirardi, M, and Seibert, M. Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms. United States: N. p., 2006.
Web.
Melis, A, Zhang, L, Benemann, J R, Forestier, M, Ghirardi, M, & Seibert, M. Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms. United States.
Melis, A, Zhang, L, Benemann, J R, Forestier, M, Ghirardi, M, and Seibert, M. Tue .
"Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms". United States.
@article{osti_894277,
title = {Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms},
author = {Melis, A and Zhang, L and Benemann, J R and Forestier, M and Ghirardi, M and Seibert, M},
abstractNote = {A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {1}
}