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Title: Method Of Signal Amplification In Multi-Chromophore Luminescence Sensors

Abstract

A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranthene (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and othermore » chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.« less

Inventors:
 [1];  [2]
  1. (Fall River, MA)
  2. (Shrewsbury, MA)
Issue Date:
Research Org.:
ALTAIR Center, LLC (Shrewsburg, MA)
OSTI Identifier:
880420
Patent Number(s):
6686206
Application Number:
10/826254
Assignee:
ALTAIR Center, LLC (Shrewsburg, MA) CHO
DOE Contract Number:  
FG02-99ER82737
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Levitsky, Igor A., and Krivoshlykov, Sergei G. Method Of Signal Amplification In Multi-Chromophore Luminescence Sensors. United States: N. p., 2004. Web.
Levitsky, Igor A., & Krivoshlykov, Sergei G. Method Of Signal Amplification In Multi-Chromophore Luminescence Sensors. United States.
Levitsky, Igor A., and Krivoshlykov, Sergei G. Tue . "Method Of Signal Amplification In Multi-Chromophore Luminescence Sensors". United States. https://www.osti.gov/servlets/purl/880420.
@article{osti_880420,
title = {Method Of Signal Amplification In Multi-Chromophore Luminescence Sensors},
author = {Levitsky, Igor A. and Krivoshlykov, Sergei G.},
abstractNote = {A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranthene (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {2}
}

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