Title: Method For Screening Microcrystallizations For Crystal Formation

Abstract

A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.

Inventors:

Santarsiero, Bernard D. ^[1]

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+ Show Inventor Affiliations

1. (Chicago, IL), Stevens, Raymond C. (La Jolla, CA), Schultz, Peter G. (La Jolla, CA), Jaklevic, Joseph M. (Lafayette, CA), Yegian, Derek T. (Oakland, CA), Cornell, Earl W. (Antioch, CA), Nordmeyer, Robert A. (San Leandro, CA)

Issue Date:

2003-10-07

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

OSTI Identifier:

879710

Patent Number(s):

6630006

Application Number:

09/851397

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J19/0046 - {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides
B01J2219/00313 - the reactor vessels being formed by arrays of wells in blocks
B01J2219/00317 - Microwell devices, i.e. having large numbers of wells
B01J2219/00328 - Movement by linear translation
B01J2219/00376 - in multiple or parallel arrangements
B01J2219/00378 - Piezo-electric or ink jet dispensers
B01J2219/00412 - In multiple arrangements
B01J2219/00547 - Bar codes
B01J2219/00585 - Parallel processes
B01J2219/0065 - by the use of liquid beads
B01J2219/00689 - using computers
B01J2219/00691 - using robots
B01J2219/00702 - Processes involving means for analysing and characterising the products
B01J2219/00725 - Peptides
B01J2219/00756 - Compositions, e.g. coatings, crystals, formulations

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2300/021 - Identification, e.g. bar codes
B01L2300/04 - Closures and closing means
B01L2300/0609 - Holders integrated in container to position an object
B01L2300/0829 - Multi-well plates
B01L2300/10 - Means to control humidity and/or other gases
B01L2400/0478 - pistons
B01L2400/0633 - with moving parts
B01L3/021 - {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids}
B01L3/0268 - {using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries}
B01L3/5085 - {for multiple samples, e.g. microtitration plates}
B01L3/50851 - {specially adapted for heating or cooling samples
B01L3/5088 - {confining liquids at a location by surface tension, e.g. virtual wells on plates, wires

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2299/00 - Coordinates from 3D structures of peptides, e.g. proteins or enzymes

C - CHEMISTRY C30 - CRYSTAL GROWTH C30B - SINGLE-CRYSTAL-GROWTH

C30B29/58 - Macromolecular compounds
C30B7/00 - Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B40/10 - Libraries containing peptides or polypeptides, or derivatives thereof
C40B60/14 - for creating libraries
C40B70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N2001/4027 - {evaporation leaving a concentrated sample}
G01N33/68 - involving proteins, peptides or amino acids {

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T436/25 - including sample preparation
Y10T436/25625 - Dilution
Y10T436/2575 - Volumetric liquid transfer

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Country of Publication:

United States

Language:

English