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Title: Method for in-situ calibration of electrophoretic analysis systems

Abstract

An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Inventors:
;
Issue Date:
Research Org.:
SpectruMedix LLC, State College, PA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
879670
Patent Number(s):
6863791
Application Number:
09/676,526
Assignee:
SpectruMedix LLC (State College, PA)
Patent Classifications (CPCs):
G - PHYSICS G06 - COMPUTING G06K - RECOGNITION OF DATA
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG02-97ER82461
Resource Type:
Patent
Resource Relation:
Patent File Date: 2000 Oct 02
Country of Publication:
United States
Language:
English

Citation Formats

Liu, Changsheng, and Zhao, Hequan. Method for in-situ calibration of electrophoretic analysis systems. United States: N. p., 2005. Web.
Liu, Changsheng, & Zhao, Hequan. Method for in-situ calibration of electrophoretic analysis systems. United States.
Liu, Changsheng, and Zhao, Hequan. Tue . "Method for in-situ calibration of electrophoretic analysis systems". United States. https://www.osti.gov/servlets/purl/879670.
@article{osti_879670,
title = {Method for in-situ calibration of electrophoretic analysis systems},
author = {Liu, Changsheng and Zhao, Hequan},
abstractNote = {An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {3}
}

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Works referenced in this record:

Automatic matrix determination in four dye fluorescence-based DNA sequencing
journal, January 1996


A method to determine the filter matrix in four-dye fluorescence-based DNA sequencing
journal, January 1997