Motorized Positioning Apparatus Having Coaxial Carrousels.
Abstract
An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
- Inventors:
-
- State College, PA
- Brooklyn, NY
- Tyrone, PA
- Loganton, PA
- Issue Date:
- Research Org.:
- Spectrumedix Corporation (State College, PA)
- OSTI Identifier:
- 879603
- Patent Number(s):
- 6365024
- Application Number:
- 09/372151
- Assignee:
- Spectrumedix Corporation (State College, PA)
- DOE Contract Number:
- FG02-97ER82461
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Li, Qingbo, Kane, Thomas E, Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V, and Kernan, John R. Motorized Positioning Apparatus Having Coaxial Carrousels.. United States: N. p., 2002.
Web.
Li, Qingbo, Kane, Thomas E, Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V, & Kernan, John R. Motorized Positioning Apparatus Having Coaxial Carrousels.. United States.
Li, Qingbo, Kane, Thomas E, Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V, and Kernan, John R. Tue .
"Motorized Positioning Apparatus Having Coaxial Carrousels.". United States. https://www.osti.gov/servlets/purl/879603.
@article{osti_879603,
title = {Motorized Positioning Apparatus Having Coaxial Carrousels.},
author = {Li, Qingbo and Kane, Thomas E and Liu, Changsheng and Sonnenschein, Bernard and Sharer, Michael V and Kernan, John R},
abstractNote = {An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {4}
}