Detector having a transmission grating beam splitter for multi-wavelength sample analysis
Abstract
A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one maymore »
- Inventors:
- Issue Date:
- Research Org.:
- Spectrumedix Corporation, State College, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 879529
- Patent Number(s):
- 5998796
- Application Number:
- 09/084,236
- Assignee:
- Spectrumedix Corporation (State College, PA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG02-97ER82461
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1998 May 26
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Liu, Changsheng, and Li, Qingbo. Detector having a transmission grating beam splitter for multi-wavelength sample analysis. United States: N. p., 1999.
Web.
Liu, Changsheng, & Li, Qingbo. Detector having a transmission grating beam splitter for multi-wavelength sample analysis. United States.
Liu, Changsheng, and Li, Qingbo. Tue .
"Detector having a transmission grating beam splitter for multi-wavelength sample analysis". United States. https://www.osti.gov/servlets/purl/879529.
@article{osti_879529,
title = {Detector having a transmission grating beam splitter for multi-wavelength sample analysis},
author = {Liu, Changsheng and Li, Qingbo},
abstractNote = {A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 07 00:00:00 EST 1999},
month = {Tue Dec 07 00:00:00 EST 1999}
}