Method To Identify Specific Inhibiutors Of Imp Dehydrogenase
Abstract
This invention relates to methods to identify specific inhibitors of the purine nucleotide synthesis enzyme, IMP dehydrogenase (IMPDH). IMPDH is an essential enzyme found in all free-living organisms from humans to bacteria and is an important therapeutic target. The invention allows the identification of specific inhibitors of any IMPDH enzyme which can be expressed in a functional form in a recombinant host cell. A variety of eukaryotic or prokaryotic host systems commonly used for the expression of recombinant proteins are suitable for the practice of the invention. The methods are amenable to high throughput systems for the screening of inhibitors generated by combinatorial chemistry or other methods such as antisense molecule production. Utilization of exogenous guanosine as a control component of the methods allows for the identification of inhibitors specific for IMPDH rather than other causes of decreased cell proliferation.
- Inventors:
-
- Bolingbrook, IL
- LaGrange, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 879487
- Patent Number(s):
- 6153398
- Application Number:
- 08/997758
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Collart, Frank R, and Huberman, Eliezer. Method To Identify Specific Inhibiutors Of Imp Dehydrogenase. United States: N. p., 2000.
Web.
Collart, Frank R, & Huberman, Eliezer. Method To Identify Specific Inhibiutors Of Imp Dehydrogenase. United States.
Collart, Frank R, and Huberman, Eliezer. Tue .
"Method To Identify Specific Inhibiutors Of Imp Dehydrogenase". United States. https://www.osti.gov/servlets/purl/879487.
@article{osti_879487,
title = {Method To Identify Specific Inhibiutors Of Imp Dehydrogenase},
author = {Collart, Frank R and Huberman, Eliezer},
abstractNote = {This invention relates to methods to identify specific inhibitors of the purine nucleotide synthesis enzyme, IMP dehydrogenase (IMPDH). IMPDH is an essential enzyme found in all free-living organisms from humans to bacteria and is an important therapeutic target. The invention allows the identification of specific inhibitors of any IMPDH enzyme which can be expressed in a functional form in a recombinant host cell. A variety of eukaryotic or prokaryotic host systems commonly used for the expression of recombinant proteins are suitable for the practice of the invention. The methods are amenable to high throughput systems for the screening of inhibitors generated by combinatorial chemistry or other methods such as antisense molecule production. Utilization of exogenous guanosine as a control component of the methods allows for the identification of inhibitors specific for IMPDH rather than other causes of decreased cell proliferation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 28 00:00:00 EST 2000},
month = {Tue Nov 28 00:00:00 EST 2000}
}
Works referenced in this record:
Cloning, sequence analysis and expression of the group A streptococcal guaB gene encoding inosine monophosphate dehydrogenase
journal, January 1995
- Ashbaugh, Cameron D.; Wessels, Michael R.
- Gene, Vol. 165, Issue 1
Characterization of human type I and type II IMP dehydrogenases.
journal, December 1993
- Carr, S. F.; Papp, E.; Wu, J. C.
- Journal of Biological Chemistry, Vol. 268, Issue 36
Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
journal, September 1988
- Amann, Egon; Ochs, Birgit; Abel, Karl-Josef
- Gene, Vol. 69, Issue 2, p. 301-315