Title: Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor

Abstract

The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.

Inventors:

Colucci, M Gabriella ^[1]; Chrispeels, Maarten J ^[2]; Moore, Jeffrey G ^[3]

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+ Show Inventor Affiliations

1. Dugenta, IT
2. La Jolla, CA
3. New York, NY

Issue Date:

2001-10-30

Research Org.:

Univ. of California (United States)

OSTI Identifier:

879431

Patent Number(s):

6310195

Application Number:

08/881189

Assignee:

ImClone Systems Incorporated (New York, NY)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K2035/124 - {the cells being hematopoietic, bone marrow derived or blood cells}
A61K38/00 - Medicinal preparations containing peptides

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P39/00 - General protective or antinoxious agents

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/42 - Lectins, e.g. concanavalin, phytohaemagglutinin

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/90 - Stable introduction of foreign DNA into chromosome
C12N2501/26 - Flt-3 ligand
C12N2501/59 - Lectins
C12N5/0647 - {Haematopoietic stem cells

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DOE Contract Number:  

FG03-86ER13497

Resource Type:

Patent

Country of Publication:

United States

Language:

English