Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor
Abstract
The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.
- Inventors:
-
- Dugenta, IT
- La Jolla, CA
- New York, NY
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 879431
- Patent Number(s):
- 6310195
- Application Number:
- 08/881189
- Assignee:
- ImClone Systems Incorporated (New York, NY)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- FG03-86ER13497
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Colucci, M Gabriella, Chrispeels, Maarten J, and Moore, Jeffrey G. Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor. United States: N. p., 2001.
Web.
Colucci, M Gabriella, Chrispeels, Maarten J, & Moore, Jeffrey G. Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor. United States.
Colucci, M Gabriella, Chrispeels, Maarten J, and Moore, Jeffrey G. Tue .
"Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor". United States. https://www.osti.gov/servlets/purl/879431.
@article{osti_879431,
title = {Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor},
author = {Colucci, M Gabriella and Chrispeels, Maarten J and Moore, Jeffrey G},
abstractNote = {The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {10}
}
Works referenced in this record:
Identification of AF-6 and Canoe as Putative Targets for Ras
journal, January 1996
- Kuriyama, Masamitsu; Harada, Naozumi; Kuroda, Shinya
- Journal of Biological Chemistry, Vol. 271, Issue 2
Heparin modulates the interaction of VEGF165 with soluble and cell associated flk-1 receptors.
journal, April 1994
- Tessler, S.; Rockwell, P.; Hicklin, D.
- Journal of Biological Chemistry, Vol. 269, Issue 17
Identification of the Protein 4.1 Binding Interface on Glycophorin C and p55, a Homologue of the Drosophila discs-large Tumor Suppressor Protein
journal, January 1995
- Marfatia, Shirin M.; Lue, Robert A.; Branton, Daniel
- Journal of Biological Chemistry, Vol. 270, Issue 2
Glycobiology: more functions for oligosaccharides
journal, September 1995
- Dwek, R.
- Science, Vol. 269, Issue 5228
Cells regulate the activities of cytokines by glycosylation
journal, March 1995
- Opdenakker, Ghislain; Rudd, Pauline M.; Wormald, Mark
- The FASEB Journal, Vol. 9, Issue 5
Lectins as cell recognition molecules
journal, October 1989
- Sharon, N.; Lis, H.
- Science, Vol. 246, Issue 4927
Functional isolation and characterization of human hematopoietic stem cells
journal, January 1995
- Berardi, A.; Wang, A.; Levine, J.
- Science, Vol. 267, Issue 5194
Growth and Differentiation in the Hemopoietic System
journal, November 1987
- Dexter, T. M.; Spooncer, E.
- Annual Review of Cell Biology, Vol. 3, Issue 1
Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro
journal, May 1996
- Shah, Aj; Smogorzewska, Em; Hannum, C.
- Blood, Vol. 87, Issue 9
Molecular cloning of Phaseolus vulgaris lectin mRNA and use of cDNA as a probe to estimate lectin transcript levels in various tissues
journal, January 1982
- Hoffman, Leslie M.; Ma, Yu; Barker, Richard F.
- Nucleic Acids Research, Vol. 10, Issue 23
STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.
journal, January 1994
- Small, D.; Levenstein, M.; Kim, E.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 2
Thrombopoietin, but not erythropoietin promotes viability and inhibits apoptosis of multipotent murine hematopoietic progenitor cells in vitro
journal, October 1996
- Borge, Oj; Ramsfjell, V.; Veiby, Op
- Blood, Vol. 88, Issue 8
The complete primary structure of a unique mannose/glucose-specific lectin from field bean (Dolichos lab lab).
journal, July 1994
- Gowda, L. R.; Savithri, H. S.; Rao, D. R.
- Journal of Biological Chemistry, Vol. 269, Issue 29
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
journal, December 1983
- Mosmann, Tim
- Journal of Immunological Methods, Vol. 65, Issue 1-2
A lectin and a lectin-related protein are the two most prominent proteins in the bark of yellow wood (Cladrastis lutea).
journal, November 1995
- Van Damme, Els J. M.; Barre, Annick; Bemer, Veronique
- Plant Molecular Biology, Vol. 29, Issue 3
Retention of quiescent hematopoietic cells with high proliferative potential during ex vivo stem cell culture
journal, January 1996
- Young, Jc; Varma, A.; DiGiusto, D.
- Blood, Vol. 87, Issue 2
A lectin gene encodes the alpha-amylase inhibitor of the common bean.
journal, October 1989
- Moreno, J.; Chrispeels, M. J.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 20
Clonal Hematopoiesis Demonstrated by X-Linked DNA Polymorphisms after Allogeneic Bone Marrow Transplantation
journal, June 1989
- Turhan, Ali G.; Humphries, R. Keith; Phillips, Gordon L.
- New England Journal of Medicine, Vol. 320, Issue 25
Non-carbohydrate binding partners/domains of animal lectins
journal, April 1994
- Gabius, Hans-Joachim
- International Journal of Biochemistry, Vol. 26, Issue 4
Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population
journal, November 1996
- Hao, Ql; Thiemann, Ft; Petersen, D.
- Blood, Vol. 88, Issue 9
The renewal and differentiation of hemopoietic stem cells
journal, June 1992
- Zipori, Dov
- The FASEB Journal, Vol. 6, Issue 9
Bifunctional properties of lectins: lectins redefined
journal, December 1988
- Barondes, Samuel H.
- Trends in Biochemical Sciences, Vol. 13, Issue 12
Tobacco Plants Transformed with the Bean α ai Gene Express an Inhibitor of Insect α-Amylase in Their Seeds
journal, June 1990
- Altabella, Teresa; Chrispeels, Maarten J.
- Plant Physiology, Vol. 93, Issue 2
Mitogenic signalling and substrate specificity of the Flk2/Flt3 receptor tyrosine kinase in fibroblasts and interleukin 3-dependent hematopoietic cells.
journal, October 1993
- Dosil, M.; Wang, S.; Lemischka, I. R.
- Molecular and Cellular Biology, Vol. 13, Issue 10
The sequence of a pea vicilin gene and its expression in transgenic tobacco plants
journal, January 1988
- Higgins, Thomas J. V.; Newbigin, Edward J.; Spencer, Donald
- Plant Molecular Biology, Vol. 11, Issue 5