skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic Acid Detection Methods

Abstract

The invention relates to methods for rapidly determining the sequence and/or length a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguish from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.

Inventors:
 [1];  [2];  [1];  [1]
  1. Boston, MA
  2. Brookline, MA
Issue Date:
Research Org.:
Boston University
Sponsoring Org.:
USDOE
OSTI Identifier:
879271
Patent Number(s):
5753439
Application Number:
08/446102
Assignee:
Trustees of Boston University (Boston, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Smith, Cassandra L, Yaar, Ron, Szafranski, Przemyslaw, and Cantor, Charles R. Nucleic Acid Detection Methods. United States: N. p., 1998. Web.
Smith, Cassandra L, Yaar, Ron, Szafranski, Przemyslaw, & Cantor, Charles R. Nucleic Acid Detection Methods. United States.
Smith, Cassandra L, Yaar, Ron, Szafranski, Przemyslaw, and Cantor, Charles R. Tue . "Nucleic Acid Detection Methods". United States. https://www.osti.gov/servlets/purl/879271.
@article{osti_879271,
title = {Nucleic Acid Detection Methods},
author = {Smith, Cassandra L and Yaar, Ron and Szafranski, Przemyslaw and Cantor, Charles R},
abstractNote = {The invention relates to methods for rapidly determining the sequence and/or length a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguish from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

Dynamic mutations: A new class of mutations causing human disease
journal, September 1992


Simple tandem DNA repeats and human genetic disease.
journal, April 1995


Advances in genosensor research
journal, May 1995


Oligonucleotide Arrays: New Concepts and Possibilities
journal, November 1994


Triplet repeat mutations in human disease
journal, May 1992


Trinucleotide repeat instability: when and where?
journal, June 1993


Two-dimensional S1 nuclease heteroduplex mapping: detection of rearrangements in bacterial genomes.
journal, May 1984


A rapid scanning strip for tri- and dinucleotide short tandem repeats
journal, January 1994


Genes with triplet repeats: candidate mediators of neuropsychiatric disorders
journal, July 1993