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Title: Dna Sequencing

A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.
 [1];  [2]
  1. (Cambridge, MA)
  2. (Chestnut Hill, MA)
Issue Date:
OSTI Identifier:
President and Fellows of Harvard College (Cambridge, MA) CHO
Patent Number(s):
US 5409811
Application Number:
Contract Number:
Research Org:
Harvard University
Country of Publication:
United States