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Title: Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus

A purified low molecular weight {beta}-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-{beta}-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65 C at a pH range of from about 2 to about 7, has an inactivation temperature of about 80 C at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5--54.5 kD as determined by SDS-PAGE. 6 figs.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
87758
Assignee:
Midwest Research Inst., Kansas City, MO (United States) PTO; SCA: 550200; PA: EDB-95:120069; SN: 95001431605
Patent Number(s):
US 5,432,075/A/
Application Number:
PAN: 8-275,995
Contract Number:
AC36-83CH10093
Resource Relation:
Other Information: PBD: 11 Jul 1995
Research Org:
Midwest Research Institute
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GLUCOSIDASE; PROTEIN ENGINEERING; BIOTECHNOLOGY; BACTERIA; ENZYME ACTIVITY; ELECTROPHORESIS