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Title: Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus

Abstract

A purified low molecular weight {beta}-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-{beta}-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65 C at a pH range of from about 2 to about 7, has an inactivation temperature of about 80 C at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5--54.5 kD as determined by SDS-PAGE. 6 figs.

Inventors:
; ; ;
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
OSTI Identifier:
87758
Patent Number(s):
5,432,075
Application Number:
PAN: 8-275,995
Assignee:
Midwest Research Inst., Kansas City, MO (United States)
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 11 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GLUCOSIDASE; PROTEIN ENGINEERING; BIOTECHNOLOGY; BACTERIA; ENZYME ACTIVITY; ELECTROPHORESIS

Citation Formats

Himmel, M E, Tucker, M P, Adney, W S, and Nieves, R A. Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus. United States: N. p., 1995. Web.
Himmel, M E, Tucker, M P, Adney, W S, & Nieves, R A. Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus. United States.
Himmel, M E, Tucker, M P, Adney, W S, and Nieves, R A. Tue . "Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus". United States.
@article{osti_87758,
title = {Low molecular weight thermostable {beta}-D-glucosidase from Acidothermus cellulolyticus},
author = {Himmel, M E and Tucker, M P and Adney, W S and Nieves, R A},
abstractNote = {A purified low molecular weight {beta}-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-{beta}-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65 C at a pH range of from about 2 to about 7, has an inactivation temperature of about 80 C at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5--54.5 kD as determined by SDS-PAGE. 6 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {7}
}