Mutant E. coli strain with increased succinic acid production
Abstract
A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.
- Inventors:
-
- Warrenville, IL
- Plainfield, IL
- Woodridge, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 875302
- Patent Number(s):
- RE37393
- Assignee:
- University of Chicago (Chicago, IL)
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- mutant; coli; strain; increased; succinic; acid; production; method; isolating; producing; bacteria; provided; comprising; increasing; biomass; organism; lacks; ability; catabolize; pyruvate; subjecting; glucose-rich; medium; anaerobic; environment; enable; pyruvate-catabolizing; mutants; grow; provides; produces; amounts; derived; parent; lacked; genes; formate; lyase; lactate; dehydrogenase; belongs; acid production; succinic acid; provided comprising; acid producing; producing bacteria; catabolize pyruvate; comprising increasing; coli strain; increased succinic; isolating succinic; /435/
Citation Formats
Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States: N. p., 2002.
Web.
Donnelly, Mark, Millard, Cynthia S, & Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States.
Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. Tue .
"Mutant E. coli strain with increased succinic acid production". United States. https://www.osti.gov/servlets/purl/875302.
@article{osti_875302,
title = {Mutant E. coli strain with increased succinic acid production},
author = {Donnelly, Mark and Millard, Cynthia S and Stols, Lucy},
abstractNote = {A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}
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