Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression
Abstract
The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
- Inventors:
-
- Richland, WA
- Pendleton, OR
- Kennewick, WA
- Pasco, WA
- Issue Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 875139
- Patent Number(s):
- 6541622
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; yeast; alpha-amylase; promoter; regulated; heterologous; expression; provides; clone; discovery; starch; utilizing; strain; schwanniomyces; castellii; isolated; inducible; regulate; strong; culture; medium; culture medium; /999/435/
Citation Formats
Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, and Anderson, Daniel B. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression. United States: N. p., 2003.
Web.
Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, & Anderson, Daniel B. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression. United States.
Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, and Anderson, Daniel B. Tue .
"Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression". United States. https://www.osti.gov/servlets/purl/875139.
@article{osti_875139,
title = {Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression},
author = {Gao, Johnway and Skeen, Rodney S and Hooker, Brian S and Anderson, Daniel B},
abstractNote = {The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {4}
}
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