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Title: Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

Abstract

The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Inventors:
 [1];  [2];  [3];  [4]
+ Show Inventor Affiliations
  1. Richland, WA
  2. Pendleton, OR
  3. Kennewick, WA
  4. Pasco, WA
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
875139
Patent Number(s):
6,541,622
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; yeast; alpha-amylase; promoter; regulated; heterologous; expression; provides; clone; discovery; starch; utilizing; strain; schwanniomyces; castellii; isolated; inducible; regulate; strong; culture; medium; culture medium; /999/435/

Citation Formats

Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, and Anderson, Daniel B. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression. United States: N. p., 2003. Web.
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Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, & Anderson, Daniel B. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression. United States.
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Gao, Johnway, Skeen, Rodney S, Hooker, Brian S, and Anderson, Daniel B. Tue . "Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression". United States. https://www.osti.gov/servlets/purl/875139.
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@article{osti_875139,
title = {Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression},
author = {Gao, Johnway and Skeen, Rodney S and Hooker, Brian S and Anderson, Daniel B},
abstractNote = {The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {4}
}
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