Microfluidic devices for the controlled manipulation of small volumes
Abstract
A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemented on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses and manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 875072
- Patent Number(s):
- 6524456
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- microfluidic; devices; controlled; manipulation; volumes; method; conducting; broad; range; biochemical; analyses; manipulations; series; nano-; subnanoliter; reaction; apparatus; carrying; disclosed; implemented; fluidic; microchip; provide; serial; throughput; device; lend; multiple; parallel; generation; information; microfabricated; channel; manipulate; nanoliter; manner; produce; results; rates; 10; hz; individual; manipulated; fashion; analogous; digital; shift; register; application; screening; molecular; cellular; targets; single; beads; split-synthesis; combinatorial; libraries; cells; rna; protein; expression; genetic; diagnostic; cell; level; performing; signal; transduction; studies; chemical reaction; single cell; reaction volume; microfluidic devices; /204/422/436/
Citation Formats
Ramsey, J Michael, and Jacobson, Stephen C. Microfluidic devices for the controlled manipulation of small volumes. United States: N. p., 2003.
Web.
Ramsey, J Michael, & Jacobson, Stephen C. Microfluidic devices for the controlled manipulation of small volumes. United States.
Ramsey, J Michael, and Jacobson, Stephen C. Tue .
"Microfluidic devices for the controlled manipulation of small volumes". United States. https://www.osti.gov/servlets/purl/875072.
@article{osti_875072,
title = {Microfluidic devices for the controlled manipulation of small volumes},
author = {Ramsey, J Michael and Jacobson, Stephen C},
abstractNote = {A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemented on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses and manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 25 00:00:00 EST 2003},
month = {Tue Feb 25 00:00:00 EST 2003}
}