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Title: Microfluidic devices for the controlled manipulation of small volumes

Abstract

A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemented on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses and manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.

Inventors:
 [1];  [1]
  1. Knoxville, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
875072
Patent Number(s):
6,524,456
Assignee:
UT-Battelle, LLC (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microfluidic; devices; controlled; manipulation; volumes; method; conducting; broad; range; biochemical; analyses; manipulations; series; nano-; subnanoliter; reaction; apparatus; carrying; disclosed; implemented; fluidic; microchip; provide; serial; throughput; device; lend; multiple; parallel; generation; information; microfabricated; channel; manipulate; nanoliter; manner; produce; results; rates; 10; hz; individual; manipulated; fashion; analogous; digital; shift; register; application; screening; molecular; cellular; targets; single; beads; split-synthesis; combinatorial; libraries; cells; rna; protein; expression; genetic; diagnostic; cell; level; performing; signal; transduction; studies; chemical reaction; single cell; reaction volume; microfluidic devices; /204/422/436/

Citation Formats

Ramsey, J Michael, and Jacobson, Stephen C. Microfluidic devices for the controlled manipulation of small volumes. United States: N. p., 2003. Web.
Ramsey, J Michael, & Jacobson, Stephen C. Microfluidic devices for the controlled manipulation of small volumes. United States.
Ramsey, J Michael, and Jacobson, Stephen C. Tue . "Microfluidic devices for the controlled manipulation of small volumes". United States. https://www.osti.gov/servlets/purl/875072.
@article{osti_875072,
title = {Microfluidic devices for the controlled manipulation of small volumes},
author = {Ramsey, J Michael and Jacobson, Stephen C},
abstractNote = {A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemented on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses and manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {2}
}

Patent:

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