skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Sol-gel method for encapsulating molecules

Abstract

A method for encapsulating organic molecules, and in particular, biomolecules using sol-gel chemistry. A silica sol is prepared from an aqueous alkali metal silicate solution, such as a mixture of silicon dioxide and sodium or potassium oxide in water. The pH is adjusted to a suitably low value to stabilize the sol by minimizing the rate of siloxane condensation, thereby allowing storage stability of the sol prior to gelation. The organic molecules, generally in solution, is then added with the organic molecules being encapsulated in the sol matrix. After aging, either a thin film can be prepared or a gel can be formed with the encapsulated molecules. Depending upon the acid used, pH, and other processing conditions, the gelation time can be from one minute up to several days. In the method of the present invention, no alcohols are generated as by-products during the sol-gel and encapsulation steps. The organic molecules can be added at any desired pH value, where the pH value is generally chosen to achieve the desired reactivity of the organic molecules. The method of the present invention thereby presents a sufficiently mild encapsulation method to retain a significant portion of the activity of the biomolecules, comparedmore » with the activity of the biomolecules in free solution.« less

Inventors:
 [1];  [1];  [1];  [2]
  1. Albuquerque, NM
  2. San Francisco, CA
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
OSTI Identifier:
874945
Patent Number(s):
6495352
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
sol-gel; method; encapsulating; molecules; organic; biomolecules; chemistry; silica; sol; prepared; aqueous; alkali; metal; silicate; solution; mixture; silicon; dioxide; sodium; potassium; oxide; water; ph; adjusted; suitably; value; stabilize; minimizing; rate; siloxane; condensation; allowing; storage; stability; prior; gelation; added; encapsulated; matrix; aging; film; formed; depending; acid; processing; conditions; time; minute; days; alcohols; generated; by-products; encapsulation; steps; chosen; achieve; reactivity; sufficiently; mild; retain; significant; portion; activity; compared; free; alkali metal; silicon dioxide; aqueous alkali; silica sol; sol-gel chemistry; organic molecule; /435/436/

Citation Formats

Brinker, C Jeffrey, Ashley, Carol S, Bhatia, Rimple, and Singh, Anup K. Sol-gel method for encapsulating molecules. United States: N. p., 2002. Web.
Brinker, C Jeffrey, Ashley, Carol S, Bhatia, Rimple, & Singh, Anup K. Sol-gel method for encapsulating molecules. United States.
Brinker, C Jeffrey, Ashley, Carol S, Bhatia, Rimple, and Singh, Anup K. Tue . "Sol-gel method for encapsulating molecules". United States. https://www.osti.gov/servlets/purl/874945.
@article{osti_874945,
title = {Sol-gel method for encapsulating molecules},
author = {Brinker, C Jeffrey and Ashley, Carol S and Bhatia, Rimple and Singh, Anup K},
abstractNote = {A method for encapsulating organic molecules, and in particular, biomolecules using sol-gel chemistry. A silica sol is prepared from an aqueous alkali metal silicate solution, such as a mixture of silicon dioxide and sodium or potassium oxide in water. The pH is adjusted to a suitably low value to stabilize the sol by minimizing the rate of siloxane condensation, thereby allowing storage stability of the sol prior to gelation. The organic molecules, generally in solution, is then added with the organic molecules being encapsulated in the sol matrix. After aging, either a thin film can be prepared or a gel can be formed with the encapsulated molecules. Depending upon the acid used, pH, and other processing conditions, the gelation time can be from one minute up to several days. In the method of the present invention, no alcohols are generated as by-products during the sol-gel and encapsulation steps. The organic molecules can be added at any desired pH value, where the pH value is generally chosen to achieve the desired reactivity of the organic molecules. The method of the present invention thereby presents a sufficiently mild encapsulation method to retain a significant portion of the activity of the biomolecules, compared with the activity of the biomolecules in free solution.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Aqueous Sol−Gel Process for Protein Encapsulation
journal, August 2000


Enzymatic activity of glucose oxidase encapsulated in transparent glass by the sol-gel method
journal, May 1992


Synthesis of Sol-Gel Matrices for Encapsulation of Enzymes Using an Aqueous Route
journal, January 1998


Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method
journal, February 1992


Encapsulation of enzymes and cells in sol-gel matrices for biosensor applications
conference, December 1999