Phytochelatin synthases and uses therefor
Abstract
The present invention relates to novel phytochelatin synthases and uses thereof.
- Inventors:
-
- Ardmore, PA
- Philadelphia, PA
- Jenkintown, PA
- Oak Park, CA
- La Jolla, CA
- San Diego, CA
- Halle, DE
- Issue Date:
- Research Org.:
- Univ. of Pennsylvania, Philadelphia, PA (United States); Univ. of California (United States)
- OSTI Identifier:
- 874919
- Patent Number(s):
- 6489537
- Assignee:
- The Trustees of the University of Pennsylvania (Philadelphia, PA); University of California (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-91ER20055; FG02-94ER20162; FG07-96ER20253
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- phytochelatin; synthases; relates; novel; /800/435/999/
Citation Formats
Rea, Philip A, Vatamaniuk, Olena K, Mari, Stephane, Lu, Yu-Ping, Schroeder, Julian I, Kim, Eugene J, and Clemens, Stephan. Phytochelatin synthases and uses therefor. United States: N. p., 2002.
Web.
Rea, Philip A, Vatamaniuk, Olena K, Mari, Stephane, Lu, Yu-Ping, Schroeder, Julian I, Kim, Eugene J, & Clemens, Stephan. Phytochelatin synthases and uses therefor. United States.
Rea, Philip A, Vatamaniuk, Olena K, Mari, Stephane, Lu, Yu-Ping, Schroeder, Julian I, Kim, Eugene J, and Clemens, Stephan. Tue .
"Phytochelatin synthases and uses therefor". United States. https://www.osti.gov/servlets/purl/874919.
@article{osti_874919,
title = {Phytochelatin synthases and uses therefor},
author = {Rea, Philip A and Vatamaniuk, Olena K and Mari, Stephane and Lu, Yu-Ping and Schroeder, Julian I and Kim, Eugene J and Clemens, Stephan},
abstractNote = {The present invention relates to novel phytochelatin synthases and uses thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants
journal, August 1994
- Schachtman, Daniel P.; Schroeder, Julian I.
- Nature, Vol. 370, Issue 6491
Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae
journal, February 1998
- Inoue, Yoshiharu; Sugiyama, Kei-ichi; Izawa, Shingo
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1395, Issue 3
Phytochelatins and Related Peptides (Structure, Biosynthesis, and Function)
journal, December 1995
- Rauser, W. E.
- Plant Physiology, Vol. 109, Issue 4
Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins
journal, January 1987
- Grill, E.; Winnacker, E. -L.; Zenk, M. H.
- Proceedings of the National Academy of Sciences, Vol. 84, Issue 2
Oxidative stress in plants
journal, January 1995
- Inzé, Dirk; Montagu, Marc Van
- Current Opinion in Biotechnology, Vol. 6, Issue 2
Review article. Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants
journal, April 1998
- Noctor, G.
- Journal of Experimental Botany, Vol. 49, Issue 321
Ribozymes and Their Medical Implications
journal, November 1988
- Cech, Thomas R.
- JAMA: The Journal of the American Medical Association, Vol. 260, Issue 20
Cadmium transport across tonoplast of vesicles from oat roots. Evidence for a Cd2+/H+ antiport activity
journal, June 1993
- Salt, D. E.; Wagner, G. J.
- Journal of Biological Chemistry, Vol. 268, Issue 17
Promises and Prospects of Phytoremediation
journal, March 1996
- Cunningham, S. D.; Ow, D. W.
- Plant Physiology, Vol. 110, Issue 3
Antisense genes in plants: an overview
journal, December 1988
- van der Krol, Alexander R.; Mol, Joseph N. M.; Stuitje, Antoine R.
- Gene, Vol. 72, Issue 1-2
Antisense RNA and DNA
journal, January 1990
- Weintraub, Harold M.
- Scientific American, Vol. 262, Issue 1
RNA catalytic properties of the minimum (-)sTRSV sequence
journal, June 1989
- Hampel, Arnold; Tritz, Richard
- Biochemistry, Vol. 28, Issue 12
Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection
journal, August 1988
- Valvekens, D.; Montagu, M. V.; Lijsebettens, M. V.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 15
A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein.
journal, September 1994
- Szczypka, M. S.; Wemmie, J. A.; Moye-Rowley, W. S.
- Journal of Biological Chemistry, Vol. 269, Issue 36
Binary Agrobacterium vectors for plant transformation
journal, January 1984
- Bevan, Michael
- Nucleic Acids Research, Vol. 12, Issue 22
The Heavy Metal-Binding Peptides of Plants
journal, June 1990
- Steffens, J. C.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 41, Issue 1
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
journal, May 1992
- Anderson, J. A.; Huprikar, S. S.; Kochian, L. V.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 9
High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans -complementation of Schizosaccharomyces pombe
journal, January 1990
- Okazaki, Koei; Okazaki, Noriko; Kume, Kazuhiko
- Nucleic Acids Research, Vol. 18, Issue 22
The Role of Glutathione in Copper Metabolism and Toxicity
journal, April 1989
- Freedman, J. H.; Ciriolo, M. R.; Peisach, J.
- Journal of Biological Chemistry, Vol. 264, Issue 10
AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: Isolation and functional definition of a plant ATP-binding cassette transporter gene
journal, July 1997
- Lu, Y. -P.; Li, Z. -S.; Rea, P. A.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 15
Synthesis of metallothionein-like peptides cadystin A and B occurring in a fission yeast, and their isomers.
journal, January 1985
- Kondo, Naoto; Isobe, Minoru; Imai, Kunio
- Agricultural and Biological Chemistry, Vol. 49, Issue 1
Transcriptional activation mediated by the yeast AP-1 protein is required for normal cadmium tolerance.
journal, May 1994
- Wemmie, J. A.; Wu, A. L.; Harshman, K. D.
- Journal of Biological Chemistry, Vol. 269, Issue 20
Peroxidase-Induced Wilting in Transgenic Tobacco Plants.
journal, January 1990
- Lagrimini, L. M.; Bradford, S.; Rothstein, S.
- The Plant Cell
Phytoremediation of contaminated soils
journal, September 1995
- Cunningham, Scott D.; Berti, William R.; Huang, Jianwei W.
- Trends in Biotechnology, Vol. 13, Issue 9
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
journal, May 1976
- Bradford, Marion M.
- Analytical Biochemistry, Vol. 72, Issue 1-2
Cadmium-Sensitive, cad1 Mutants of Arabidopsis thaliana Are Phytochelatin Deficient
journal, April 1995
- Howden, R.; Goldsbrough, P. B.; Andersen, C. R.
- Plant Physiology, Vol. 107, Issue 4
Modification of thiol contents in poplars ( Populus tremula × P. alba ) overexpressing enzymes involved in glutathione synthesis
journal, October 1997
- Arisi, Ana-Carolina M.; Noctor, Graham; Foyer, Christine H.
- Planta, Vol. 203, Issue 3
Transport of Metal-binding Peptides by HMT1, A Fission Yeast ABC-type Vacuolar Membrane Protein
journal, March 1995
- Ortiz, Daniel F.; Ruscitti, Theresa; McCue, Kent F.
- Journal of Biological Chemistry, Vol. 270, Issue 9
Simple RNA enzymes with new and highly specific endoribonuclease activities
journal, August 1988
- Haseloff, Jim; Gerlach, Wayne L.
- Nature, Vol. 334, Issue 6183
Reactivation of metal-requiring apoenzymes by phytochelatin-metal complexes
journal, June 1991
- Thumann, Josef; Grill, Erwin; Winnacker, Ernst-L.
- FEBS Letters, Vol. 284, Issue 1
A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation.
journal, December 1991
- Robinson, J. S.; Graham, T. R.; Emr, S. D.
- Molecular and Cellular Biology, Vol. 11, Issue 12
Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase: BLOCKED THIOLS ARE SUFFICIENT FOR PC SYNTHASE-CATALYZED TRANSPEPTIDATION OF GLUTATHIONE AND RELATED THIOL PEPTIDES
journal, May 2000
- Vatamaniuk, Olena K.; Mari, Stéphane; Lu, Yu-Ping
- Journal of Biological Chemistry, Vol. 275, Issue 40
Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter.
journal, October 1992
- Ortiz, D. F.; Kreppel, L.; Speiser, D. M.
- The EMBO Journal, Vol. 11, Issue 10
Phytochelatins: The Principal Heavy-Metal Complexing Peptides of Higher Plants
journal, November 1985
- Grill, E.; Winnacker, E. -L.; Zenk, M. H.
- Science, Vol. 230, Issue 4726
Plant genetic engineering may help with environmental cleanup.
journal, April 1996
- Raskin, I.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
The Yeast Cadmium Factor Protein (YCF1) Is a Vacuolar Glutathione S-Conjugate Pump
journal, March 1996
- Li, Ze-Sheng; Szczypka, Mark; Lu, Yu-Ping
- Journal of Biological Chemistry, Vol. 271, Issue 11
Phytochelatins
journal, June 1990
- Rauser, Wilfried E.
- Annual Review of Biochemistry, Vol. 59, Issue 1
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer
journal, December 1988
- Higgins, Desmond G.; Sharp, Paul M.
- Gene, Vol. 73, Issue 1, p. 237-244
A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium
journal, January 1997
- Li, Z. -S.; Lu, Y. -P.; Zhen, R. -G.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 1
Techniques for using antisense oligodeoxyribonucleotides to study gene expression
journal, August 1988
- Marcus-Sekura, Carol J.
- Analytical Biochemistry, Vol. 172, Issue 2, p. 289-295
Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
journal, January 1990
- Stadtman, Earl R.
- Free Radical Biology and Medicine, Vol. 9, Issue 4
From Vacuolar gs-x Pumps to Multispecific abc Transporters
journal, June 1998
- Rea, Philip A.; Li, Ze-Sheng; Lu, Yu-Ping
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 49, Issue 1
Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin
journal, April 1992
- Kneer, Ralf; Kutchan, Toni M.; Hochberger, Andreas
- Archives of Microbiology, Vol. 157, Issue 4
Function and autoregulation of yeast copperthionein
journal, May 1985
- Hamer, D.; Thiele, D.; Lemontt, J.
- Science, Vol. 228, Issue 4700
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans
journal, April 1990
- Napoli, Carolyn; Lemieux, Christine; Jorgensen, Richard
- The Plant Cell, Vol. 2, Issue 4
Characterization of phytochelatin synthase from tomato
journal, September 1997
- Chen, Jianjun; Zhou, Jianmin; Goldsbrough, Peter B.
- Physiologia Plantarum, Vol. 101, Issue 1
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987
- Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W.
- The EMBO Journal, Vol. 6, Issue 13
[21] Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors
book, January 1991
- Strathern, Jeffrey N.; Higgins, David R.
- Guide to Yeast Genetics and Molecular Biology
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
journal, November 1972
- Udenfriend, S.; Stein, S.; Bohlen, P.
- Science, Vol. 178, Issue 4063
Isolation of mutants of Schizosaccharomycespombe unable to synthesize cadystin, small cadmium-binding peptides
journal, February 1988
- Mutoh, Norihiro; Hayashi, Yukimasa
- Biochemical and Biophysical Research Communications, Vol. 151, Issue 1
Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier
journal, April 1991
- Gietz, R. Daniel; Schiestl, Robert H.
- Yeast, Vol. 7, Issue 3
Nuclear magnetic resonance studies of the solution chemistry of metal complexes. IX. Binding of cadmium, zinc, lead, and mercury by glutathione
journal, October 1973
- Fuhr, Bryan J.; Rabenstein, Dallas L.
- Journal of the American Chemical Society, Vol. 95, Issue 21
Fast forward genetics based on virus-induced gene silencing
journal, April 1999
- Baulcombe, David C.
- Current Opinion in Plant Biology, Vol. 2, Issue 2
Quantitative assessment of worldwide contamination of air, water and soils by trace metals
journal, May 1988
- Nriagu, Jerome O.; Pacyna, Jozef M.
- Nature, Vol. 333, Issue 6169
Purification and Immunological Identification of Metallothioneins 1 and 2 from Arabidopsis thaliana
journal, April 1997
- Murphy, A.; Zhou, J.; Goldsbrough, P. B.
- Plant Physiology, Vol. 113, Issue 4
Heterologous expression of plant vacuolar pyrophosphatase in yeast demonstrates sufficiency of the substrate-binding subunit for proton transport.
journal, June 1994
- Kim, E. J.; Zhen, R. G.; Rea, P. A.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 13
Subcellular Localization of Cadmium and Cadmium-Binding Peptides in Tobacco Leaves: Implication of a Transport Function for Cadmium-Binding Peptides
journal, April 1990
- Vögeli-Lange, Regina; Wagner, George J.
- Plant Physiology, Vol. 92, Issue 4
Heavy metal detoxification in higher plants - a review
journal, January 1996
- Zenk, Meinhart H.
- Gene, Vol. 179, Issue 1
The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast
journal, September 1998
- Clemens, S.; Antosiewicz, D. M.; Ward, J. M.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 20
Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific -glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase)
journal, September 1989
- Grill, E.; Loffler, S.; Winnacker, E. -L.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 18
Overview of the yeast genome
journal, May 1997
- Mewes, H. W.; Albermann, K.; Bähr, M.
- Nature, Vol. 387, Issue S6632
Three families of thiol peptides are induced by cadmium in maize
journal, March 1995
- Meuwly, Philippe; Thibault, Pierre; Schwan, Adrian L.
- The Plant Journal, Vol. 7, Issue 3
BACTERIAL HEAVY METAL RESISTANCE: New Surprises
journal, October 1996
- Silver, Simon; Phung, Le T.
- Annual Review of Microbiology, Vol. 50, Issue 1
Functional homologs of fungal metallothionein genes from Arabidopsis.
journal, June 1994
- Zhou, J.; Goldsbrough, P. B.
- The Plant Cell, Vol. 6, Issue 6
Two pathways in the biosynthesis of cadystins (γEC) n G in the cell-free system of the fission yeast
journal, February 1991
- Hayashi, Yukimasa; Nakagawa, Chiaki W.; Mutoh, Norihiro
- Biochemistry and Cell Biology, Vol. 69, Issue 2-3
Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase.
journal, January 1993
- Ho, M. N.; Hill, K. J.; Lindorfer, M. A.
- Journal of Biological Chemistry, Vol. 268, Issue 1
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
- Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
- Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
Comparison of Metallothionein Gene Expression and Nonprotein Thiols in Ten Arabidopsis Ecotypes (Correlation with Copper Tolerance)
journal, November 1995
- Murphy, A.; Taiz, L.
- Plant Physiology, Vol. 109, Issue 3
RNA catalysis by a group I ribozyme. Developing a model for transition state stabilization.
journal, September 1992
- Cech, T. R.; Herschlag, D.; Piccirilli, J. A.
- Journal of Biological Chemistry, Vol. 267, Issue 25
Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.
journal, April 1990
- van der Krol, A. R.; Mur, L. A.; Beld, M.
- The Plant Cell, Vol. 2, Issue 4
Dual system for potassium transport in Saccharomyces cerevisiae.
journal, January 1984
- Rodríguez-Navarro, A.; Ramos, J.
- Journal of Bacteriology, Vol. 159, Issue 3