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Title: System and method for a parallel immunoassay system

Abstract

A method and system for detecting a target antigen using massively parallel immunoassay technology. In this system, high affinity antibodies of the antigen are covalently linked to small beads or particles. The beads are exposed to a solution containing DNA-oligomer-mimics of the antigen. The mimics which are reactive with the covalently attached antibody or antibodies will bind to the appropriate antibody molecule on the bead. The particles or beads are then washed to remove any unbound DNA-oligomer-mimics and are then immobilized or trapped. The bead-antibody complexes are then exposed to a test solution which may contain the targeted antigens. If the antigen is present it will replace the mimic since it has a greater affinity for the respective antibody. The particles are then removed from the solution leaving a residual solution. This residual solution is applied a DNA chip containing many samples of complimentary DNA. If the DNA tag from a mimic binds with its complimentary DNA, it indicates the presence of the target antigen. A flourescent tag can be used to more easily identify the bound DNA tag.

Inventors:
 [1]
  1. Naperville, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
874915
Patent Number(s):
6489120
Assignee:
The United States of America as represented by the United States Department (Washington, DC)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; parallel; immunoassay; detecting; target; antigen; massively; technology; affinity; antibodies; covalently; linked; beads; particles; exposed; solution; containing; dna-oligomer-mimics; mimics; reactive; attached; antibody; bind; appropriate; molecule; bead; washed; remove; unbound; immobilized; trapped; bead-antibody; complexes; contain; targeted; antigens; replace; mimic; removed; leaving; residual; applied; dna; chip; samples; complimentary; tag; binds; indicates; presence; flourescent; easily; identify; bound; massively parallel; /435/999/

Citation Formats

Stevens, Fred J. System and method for a parallel immunoassay system. United States: N. p., 2002. Web.
Stevens, Fred J. System and method for a parallel immunoassay system. United States.
Stevens, Fred J. Tue . "System and method for a parallel immunoassay system". United States. https://www.osti.gov/servlets/purl/874915.
@article{osti_874915,
title = {System and method for a parallel immunoassay system},
author = {Stevens, Fred J},
abstractNote = {A method and system for detecting a target antigen using massively parallel immunoassay technology. In this system, high affinity antibodies of the antigen are covalently linked to small beads or particles. The beads are exposed to a solution containing DNA-oligomer-mimics of the antigen. The mimics which are reactive with the covalently attached antibody or antibodies will bind to the appropriate antibody molecule on the bead. The particles or beads are then washed to remove any unbound DNA-oligomer-mimics and are then immobilized or trapped. The bead-antibody complexes are then exposed to a test solution which may contain the targeted antigens. If the antigen is present it will replace the mimic since it has a greater affinity for the respective antibody. The particles are then removed from the solution leaving a residual solution. This residual solution is applied a DNA chip containing many samples of complimentary DNA. If the DNA tag from a mimic binds with its complimentary DNA, it indicates the presence of the target antigen. A flourescent tag can be used to more easily identify the bound DNA tag.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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