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Title: Method for altering antibody light chain interactions

Abstract

A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.

Inventors:
 [1];  [2];  [3];  [4]
  1. Naperville, IL
  2. Evanston, IL
  3. Elmhurst, IL
  4. Downers Grove, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
874898
Patent Number(s):
6485943
Assignee:
The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; altering; antibody; light; chain; interactions; recombinant; subunit; dimerization; including; modifying; codon; nucleic; acid; sequence; replace; amino; occurring; naturally; charged; position; interface; segment; polypeptide; variable; region; polarity; heavy; opposite; sequences; code; novel; proteins; latter; conjunction; inventive; provided; nucleic acid; amino acid; acid sequence; inventive method; /435/530/

Citation Formats

Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, and Schiffer, Marianne. Method for altering antibody light chain interactions. United States: N. p., 2002. Web.
Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, & Schiffer, Marianne. Method for altering antibody light chain interactions. United States.
Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, and Schiffer, Marianne. Tue . "Method for altering antibody light chain interactions". United States. https://www.osti.gov/servlets/purl/874898.
@article{osti_874898,
title = {Method for altering antibody light chain interactions},
author = {Stevens, Fred J and Stevens, Priscilla Wilkins and Raffen, Rosemarie and Schiffer, Marianne},
abstractNote = {A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

Novel arrangement of immunoglobulin variable domains: x-ray crystallographic analysis of the .lambda.-chain dimer Bence-Jones protein Loc
journal, August 1985


Self-association of human immunoglobulin kappa I light chains: role of the third hypervariable region.
journal, February 1980


Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant
journal, June 1992


Bacterial expression of immunoglobulin fragments
journal, January 1993


Reengineering immunoglobulin domain interactions by introduction of charged residues
journal, April 1998


Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems
journal, June 1991


Renaturation, Purification and Characterization of Recombinant Fab-Fragments Produced in Escherichia coli
journal, February 1991