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Title: Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17

Abstract

Methods and compositions for staining based upon nucleic acid sequence that employ nudeic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

Inventors:
 [1];  [2];  [3];  [3];  [4]
  1. Livermore, CA
  2. Walnut Creek, CA
  3. Tampere, FI
  4. Tokyo, JP
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874859
Patent Number(s):
6475720
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
chromosome-specific; staining; detect; genetic; rearrangements; associated; chromosome; andor; 17; methods; compositions; based; nucleic; acid; sequence; employ; nudeic; probes; provided; produce; patterns; tailored; specific; cytogenetic; analyses; appropriate; situ; hybridization; stain; interphase; metaphase; chromosomal; material; reliable; signals; typically; complexity; 50; kb; depending; application; reagents; detection; kits; detecting; tumor; cytogenetics; disease; related; loci; specifically; cancer; chronic; myelogenous; leukemia; cml; retinoblastoma; ovarian; uterine; cancers; biological; dosimetry; described; differentiation; cytogenetically; similar; genetically; diseases; prognostic; diagnostic; applications; nucleic acid; acid sequence; situ hybridization; chromosome-specific staining; acid probe; /435/436/999/

Citation Formats

Gray, Joe W, Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, and Sakamoto, Masaru. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17. United States: N. p., 2002. Web.
Gray, Joe W, Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, & Sakamoto, Masaru. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17. United States.
Gray, Joe W, Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, and Sakamoto, Masaru. Tue . "Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17". United States. https://www.osti.gov/servlets/purl/874859.
@article{osti_874859,
title = {Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17},
author = {Gray, Joe W and Pinkel, Daniel and Kallioniemi, Olli-Pekka and Kallioniemi, Anne and Sakamoto, Masaru},
abstractNote = {Methods and compositions for staining based upon nucleic acid sequence that employ nudeic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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