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Title: Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction

Abstract

A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.

Inventors:
 [1];  [2];  [3];  [4];  [1];  [5];  [1]
  1. (Moscow, RU)
  2. (Zelenograd, RU)
  3. (Hinsale, IL)
  4. (Western Springs, IL)
  5. (Samara, RU)
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
874803
Patent Number(s):
6465174
Assignee:
University of Chicago (Chicago, IL) ANL
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
rapid; method; detect; duplex; formation; sequencing; hybridization; methods; constructing; containment; structures; reagent; interaction; determining; existence; duplexes; oligonucleotide; complementary; molecules; provided; plurality; immobilized; specific; length; base; sequence; contacted; single; stranded; form; facilitate; intercalation; fluorescent; dye; planes; provides; matrices; comprising; confining; light; sensitive; fluid; surface; exposing; light-sensitive; pattern; exposed; polymerize; discrete; units; adhere; contacting; set; allow; disperse; base sequence; light sensitive; hybridization methods; /435/427/999/

Citation Formats

Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V., Proudnikov, Dmitri Y., and Timofeev, Edward N. Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction. United States: N. p., 2002. Web.
Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V., Proudnikov, Dmitri Y., & Timofeev, Edward N. Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction. United States.
Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V., Proudnikov, Dmitri Y., and Timofeev, Edward N. Tue . "Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction". United States. https://www.osti.gov/servlets/purl/874803.
@article{osti_874803,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction},
author = {Mirzabekov, Andrei Darievich and Yershov, Gennadiy Moiseyevich and Guschin, Dmitry Yuryevich and Gemmell, Margaret Anne and Shick, Valentine V. and Proudnikov, Dmitri Y. and Timofeev, Edward N.},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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