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Title: Triplex in-situ hybridization

Abstract

Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occurred.

Inventors:
;
Issue Date:
Research Org.:
Princeton Univ., NJ (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
874795
Patent Number(s):
6461810
Application Number:
09/531,000
Assignee:
Princeton University (Princeton, NJ)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-96ER62202
Resource Type:
Patent
Resource Relation:
Patent File Date: 2000 Sep 08
Country of Publication:
United States
Language:
English
Subject:
/435/999/

Citation Formats

Fresco, Jacques R., and Johnson, Marion D. Triplex in-situ hybridization. United States: N. p., 2002. Web.
Fresco, Jacques R., & Johnson, Marion D. Triplex in-situ hybridization. United States.
Fresco, Jacques R., and Johnson, Marion D. Tue . "Triplex in-situ hybridization". United States. https://www.osti.gov/servlets/purl/874795.
@article{osti_874795,
title = {Triplex in-situ hybridization},
author = {Fresco, Jacques R. and Johnson, Marion D.},
abstractNote = {Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occurred.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

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