Title: Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable

Abstract

This invention relates to using customized oligonucleotide microchips as biosensors for the detection and identification of nucleic acids specific for different genes, organisms and/or individuals in the environment, in food and in biological samples. The microchips are designed to convert multiple bits of genetic information into simpler patterns of signals that are interpreted as a unit. Because of an improved method of hybridizing oligonucleotides from samples to microchips, microchips are reusable and transportable. For field study, portable laser or bar code scanners are suitable.

Inventors:

Mirzabekov, Andrei ^[1]; Guschin, Dmitry Y ^[2]; Chik, Valentine ^[3]; Drobyshev, Aleksei ^[4]; Fotin, Alexander ^[5]; Yershov, Gennadiy ^[6]; Lysov, Yuri ^[6]

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+ Show Inventor Affiliations

1. Darien, IL
2. Rockville, MD
3. Woodridge, IL
4. Elektrosol, RU
5. Cambridge, MA
6. Hinsdale, IL

Issue Date:

2002-01-01

Research Org.:

Argonne National Laboratory (ANL), Argonne, IL (United States)

OSTI Identifier:

874779

Patent Number(s):

6458584

Assignee:

University of Chicago (Chicago, IL)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00644 - the porous medium being present in discrete locations, e.g. gel pads

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6837 - using probe arrays or probe chips
C12Q1/6881 - for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q1/6883 - for diseases caused by alterations of genetic material
C12Q1/689 - for bacteria
C12Q2565/513 - characterised by the pattern of the arrayed oligonucleotides
C12Q2565/607 - being a sensor, e.g. electrode
C12Q2600/156 - Polymorphic or mutational markers

G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

G16B25/00 - ICT specially adapted for hybridisation
G16B25/30 - Microarray design

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DOE Contract Number:  

W-31109-ENG-38

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

customized; oligonucleotide; microchips; convert; multiple; genetic; information; simple; patterns; portable; reusable; relates; biosensors; detection; identification; nucleic; acids; specific; genes; organisms; andor; individuals; environment; food; biological; samples; designed; bits; simpler; signals; interpreted; unit; improved; method; hybridizing; oligonucleotides; transportable; field; study; laser; bar; code; scanners; nucleic acid; /435/999/