Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments
Abstract
The present invention is directed to a method of screening large, complex, polyexonic eukaryotic genes such as the ATM gene for mutations and polymorphisms by an improved version of single strand conformation polymorphism (SSCP) electrophoresis that allows electrophoresis of two or three amplified segments in a single lane. The present invention also is directed to new mutations and polymorphisms in the ATM gene that are useful in performing more accurate screening of human DNA samples for mutations and in distinguishing mutations from polymorphisms, thereby improving the efficiency of automated screening methods.
- Inventors:
-
- Sherman Oaks, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 874778
- Patent Number(s):
- 6458536
- Application Number:
- 09/360,416
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG03-87ER60548
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- modified; sscp; method; sequential; electrophoresis; multiple; nucleic; acid; segments; directed; screening; complex; polyexonic; eukaryotic; genes; atm; mutations; polymorphisms; improved; version; single; strand; conformation; polymorphism; allows; amplified; lane; useful; performing; accurate; human; dna; samples; distinguishing; improving; efficiency; automated; methods; nucleic acid; acid segment; /435/
Citation Formats
Gatti, Richard A. Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments. United States: N. p., 2002.
Web.
Gatti, Richard A. Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments. United States.
Gatti, Richard A. Tue .
"Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments". United States. https://www.osti.gov/servlets/purl/874778.
@article{osti_874778,
title = {Modified SSCP method using sequential electrophoresis of multiple nucleic acid segments},
author = {Gatti, Richard A},
abstractNote = {The present invention is directed to a method of screening large, complex, polyexonic eukaryotic genes such as the ATM gene for mutations and polymorphisms by an improved version of single strand conformation polymorphism (SSCP) electrophoresis that allows electrophoresis of two or three amplified segments in a single lane. The present invention also is directed to new mutations and polymorphisms in the ATM gene that are useful in performing more accurate screening of human DNA samples for mutations and in distinguishing mutations from polymorphisms, thereby improving the efficiency of automated screening methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {10}
}
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