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Title: Amplification of chromosomal DNA in situ

Abstract

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Inventors:
 [1];  [2];  [2]
  1. (Tracy, CA)
  2. (Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874650
Patent Number(s):
6432650
Assignee:
The Regents of the University of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
amplification; chromosomal; dna; situ; increase; amount; associated; chromosome; region; described; provides; synthesis; fluorescence; hybridization; fish; painting; probes; single; dissected; fragments; production; cdna; libraries; copy; mrnas; improved; comparative; genomic; cgh; procedures; situ hybridization; /435/999/

Citation Formats

Christian, Allen T., Coleman, Matthew A., and Tucker, James D. Amplification of chromosomal DNA in situ. United States: N. p., 2002. Web.
Christian, Allen T., Coleman, Matthew A., & Tucker, James D. Amplification of chromosomal DNA in situ. United States.
Christian, Allen T., Coleman, Matthew A., and Tucker, James D. Tue . "Amplification of chromosomal DNA in situ". United States. https://www.osti.gov/servlets/purl/874650.
@article{osti_874650,
title = {Amplification of chromosomal DNA in situ},
author = {Christian, Allen T. and Coleman, Matthew A. and Tucker, James D.},
abstractNote = {Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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