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Title: Amplification of chromosomal DNA in situ

Abstract

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Inventors:
 [1];  [2];  [2]
  1. Tracy, CA
  2. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874650
Patent Number(s):
6432650
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
amplification; chromosomal; dna; situ; increase; amount; associated; chromosome; region; described; provides; synthesis; fluorescence; hybridization; fish; painting; probes; single; dissected; fragments; production; cdna; libraries; copy; mrnas; improved; comparative; genomic; cgh; procedures; situ hybridization; /435/999/

Citation Formats

Christian, Allen T, Coleman, Matthew A, and Tucker, James D. Amplification of chromosomal DNA in situ. United States: N. p., 2002. Web.
Christian, Allen T, Coleman, Matthew A, & Tucker, James D. Amplification of chromosomal DNA in situ. United States.
Christian, Allen T, Coleman, Matthew A, and Tucker, James D. Tue . "Amplification of chromosomal DNA in situ". United States. https://www.osti.gov/servlets/purl/874650.
@article{osti_874650,
title = {Amplification of chromosomal DNA in situ},
author = {Christian, Allen T and Coleman, Matthew A and Tucker, James D},
abstractNote = {Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

Preparative in Situ Hybridization: Selection of Chromosome Region-Specific Libraries on Mitotic Chromosomes
journal, February 1994


Identification of region specific genes by chromosome microdissection
journal, January 1997


Generation of chromosome fragment specific bovine DNA sequences by microdissection and DOP-PCR
journal, April 1996


Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992


Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992


A simple and efficient method for microdissection and microFISH.
journal, April 1998


Normalization and subtraction: two approaches to facilitate gene discovery.
journal, September 1996


PCR in situ followed by microdissection allows whole chromosome painting probes to be made from single microdissected chromosomes
journal, June 1999


Isolation of chromosome-specific ESTs by microdissection-mediated cDNA capture.
journal, February 1997