Amplification of chromosomal DNA in situ
Abstract
Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.
- Inventors:
-
- Tracy, CA
- Livermore, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 874650
- Patent Number(s):
- 6432650
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- amplification; chromosomal; dna; situ; increase; amount; associated; chromosome; region; described; provides; synthesis; fluorescence; hybridization; fish; painting; probes; single; dissected; fragments; production; cdna; libraries; copy; mrnas; improved; comparative; genomic; cgh; procedures; situ hybridization; /435/999/
Citation Formats
Christian, Allen T, Coleman, Matthew A, and Tucker, James D. Amplification of chromosomal DNA in situ. United States: N. p., 2002.
Web.
Christian, Allen T, Coleman, Matthew A, & Tucker, James D. Amplification of chromosomal DNA in situ. United States.
Christian, Allen T, Coleman, Matthew A, and Tucker, James D. Tue .
"Amplification of chromosomal DNA in situ". United States. https://www.osti.gov/servlets/purl/874650.
@article{osti_874650,
title = {Amplification of chromosomal DNA in situ},
author = {Christian, Allen T and Coleman, Matthew A and Tucker, James D},
abstractNote = {Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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