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Title: Replica amplification of nucleic acid arrays

Abstract

A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.

Inventors:
 [1]
  1. (Brookline, MA)
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
874646
Patent Number(s):
6432360
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
DOE Contract Number:  
FG02-87ER60565
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
replica; amplification; nucleic; acid; arrays; method; producing; plurality; array; comprising; steps; amplifying; situ; molecules; randomly-patterned; immobilized; heterogeneous; pool; affixed; support; transferring; subset; produced; affixing; transferred; form; occupy; positions; amplified; serves; template; produce; disclosed; nucleic acid; acid molecule; /422/435/999/

Citation Formats

Church, George M. Replica amplification of nucleic acid arrays. United States: N. p., 2002. Web.
Church, George M. Replica amplification of nucleic acid arrays. United States.
Church, George M. Tue . "Replica amplification of nucleic acid arrays". United States. https://www.osti.gov/servlets/purl/874646.
@article{osti_874646,
title = {Replica amplification of nucleic acid arrays},
author = {Church, George M.},
abstractNote = {A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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