Replica amplification of nucleic acid arrays
Abstract
A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.
- Inventors:
-
- Brookline, MA
- Issue Date:
- Research Org.:
- Harvard University
- OSTI Identifier:
- 874646
- Patent Number(s):
- 6432360
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-87ER60565
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- replica; amplification; nucleic; acid; arrays; method; producing; plurality; array; comprising; steps; amplifying; situ; molecules; randomly-patterned; immobilized; heterogeneous; pool; affixed; support; transferring; subset; produced; affixing; transferred; form; occupy; positions; amplified; serves; template; produce; disclosed; nucleic acid; acid molecule; /422/435/999/
Citation Formats
Church, George M. Replica amplification of nucleic acid arrays. United States: N. p., 2002.
Web.
Church, George M. Replica amplification of nucleic acid arrays. United States.
Church, George M. Tue .
"Replica amplification of nucleic acid arrays". United States. https://www.osti.gov/servlets/purl/874646.
@article{osti_874646,
title = {Replica amplification of nucleic acid arrays},
author = {Church, George M},
abstractNote = {A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}
Works referenced in this record:
Colony hybridization method for screening in situ of eukaryotic amplified genes
journal, May 1984
- Malpièce, Y.; Michel, M. L.; Carloni, G.
- Experientia, Vol. 40, Issue 5