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Title: DNA attachment to support structures

Abstract

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

Inventors:
 [1];  [2]
  1. Livermore, CA
  2. Fresno, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874582
Patent Number(s):
6420112
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dna; attachment; support; structures; microscopic; beads; attached; nucleic; acids; terminal; transferase; adds; labeled; dideoxy; nucleotide; bases; linear; strands; labels; antigens; digoxigenin; biotin; bind; antibody; compounds; appropriate; complementary; ligands; bound; method; require; synthesis; synthetic; oligonucleotide; probe; tag; label; sequence; blunt; fragment; >500; kilobase; pairs; nucleic acid; /435/436/530/999/

Citation Formats

Balhorn, Rodney L, and Barry, Christopher H. DNA attachment to support structures. United States: N. p., 2002. Web.
Balhorn, Rodney L, & Barry, Christopher H. DNA attachment to support structures. United States.
Balhorn, Rodney L, and Barry, Christopher H. Tue . "DNA attachment to support structures". United States. https://www.osti.gov/servlets/purl/874582.
@article{osti_874582,
title = {DNA attachment to support structures},
author = {Balhorn, Rodney L and Barry, Christopher H},
abstractNote = {Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

Behavior of Supercoiled DNA
journal, April 1998


Ionic effects on the elasticity of single DNA molecules
journal, June 1997


Deoxynucleotide-polymerizing Enzymes of Calf Thymus Gland
journal, June 1967


Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads
journal, November 1992