skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High density array fabrication and readout method for a fiber optic biosensor

Abstract

The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers aremore » then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.« less

Inventors:
 [1];  [2];  [3]
  1. Walnut Creek, CA
  2. San Francisco, CA
  3. Lafayette, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
874570
Patent Number(s):
6417506
Assignee:
The Regents of the University of California (Oakland, CA); Medical Research Council (London, GB)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
density; array; fabrication; readout; method; fiber; optic; biosensor; relates; biosensors; comprising; plurality; optical; fibers; attached; sensor; biological; binding; partners; molecules; specifically; bind; form; complex; antibody-antigen; lectin-carbohydrate; nucleic; acid-nucleic; acid; biotin-avidin; etc; bears; species; partner; fabricated; providing; treated; batch; attach; bundle; uniquely; identified; combined; discretely; addressed; selected; separated; bundles; produce; capable; assaying; simultaneously; components; sample; transmission; detectors-such; multiplicity; sensors; signal; produced; substrate; conducted; detector; discrete; examining; transmit; unique; patterns; assisting; rapid; identification; nucleic acid; optical fiber; fiber optic; optical sensor; density array; /250/204/600/

Citation Formats

Pinkel, Daniel, Gray, Joe, and Albertson, Donna G. High density array fabrication and readout method for a fiber optic biosensor. United States: N. p., 2002. Web.
Pinkel, Daniel, Gray, Joe, & Albertson, Donna G. High density array fabrication and readout method for a fiber optic biosensor. United States.
Pinkel, Daniel, Gray, Joe, and Albertson, Donna G. Tue . "High density array fabrication and readout method for a fiber optic biosensor". United States. https://www.osti.gov/servlets/purl/874570.
@article{osti_874570,
title = {High density array fabrication and readout method for a fiber optic biosensor},
author = {Pinkel, Daniel and Gray, Joe and Albertson, Donna G},
abstractNote = {The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Fiber-optic-based biosensor: signal enhancement in a production model
conference, April 1992


Fiber-optic-based biosensor: signal enhancement in a production model
conference, April 1992


Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction
journal, January 1994


Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction
journal, January 1994


Fiber-Optic DNA Sensor for Fluorometric Nucleic Acid Determination
journal, August 1995


Fiber-Optic DNA Sensor for Fluorometric Nucleic Acid Determination
journal, August 1995


An optical biosensor using a fluorescent, swelling sensing element
journal, January 1994


An optical biosensor using a fluorescent, swelling sensing element
journal, January 1994


Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
journal, September 1980


Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
journal, September 1980


Enzyme-based fiber-optic device for the determination of total and free cholesterol
journal, January 1992


Enzyme-based fiber-optic device for the determination of total and free cholesterol
journal, January 1992


Intelligent Materials Properties of DNA and Strategies for Its Incorporation into Electroactive Polymeric Thin Film Systems
journal, July 1994


Intelligent Materials Properties of DNA and Strategies for Its Incorporation into Electroactive Polymeric Thin Film Systems
journal, July 1994


One-step immobilization of immunoglobulin G and potential of the method for application in immunosensors
journal, August 1995


Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992


Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992


Cellulose As A New Matrix For One-step Immobilized Antibodies And Application In High Specific Immunosensors
conference, January 1995


A fibre-optic chemical sensor with discrete sensing sites
journal, September 1991


A fibre-optic chemical sensor with discrete sensing sites
journal, September 1991


Fiber optic biosensor for fluorimetric detection of DNA hybridization
journal, April 1994


Fiber optic biosensor for fluorimetric detection of DNA hybridization
journal, April 1994


An improved method to create nitrocellulose particles suitable for the immobilization of antigen and antibody
journal, November 1995


An improved method to create nitrocellulose particles suitable for the immobilization of antigen and antibody
journal, November 1995


Continuous-flow bioluminescent assay of NADH using a fibre-optic sensor
journal, November 1989


Continuous-flow bioluminescent assay of NADH using a fibre-optic sensor
journal, November 1989


Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992


Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992


Luminescence Fiber-Optic Biosensor
journal, May 1988


Luminescence Fiber-Optic Biosensor
journal, May 1988


Multifiber, multiwavelength, fiber optic fluorescence spectrophotometer
journal, January 1992


Multifiber, multiwavelength, fiber optic fluorescence spectrophotometer
journal, January 1992


Fluorescence microstructure using a laser/fiber optic profiler
conference, September 1990


Fluorescence microstructure using a laser/fiber optic profiler
conference, September 1990