skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Polynucleotides encoding TRF1 binding proteins

Abstract

The present invention provides a novel telomere associated protein (Trf1-interacting nuclear protein 2 "Tin2") that hinders the binding of Trf1 to its specific telomere repeat sequence and mediates the formation of a Tin2-Trf1-telomeric DNA complex that limits telomerase access to the telomere. Also included are the corresponding nucleic acids that encode the Tin2 of the present invention, as well as mutants of Tin2. Methods of making, purifying and using Tin2 of the present invention are described. In addition, drug screening assays to identify drugs that mimic and/or complement the effect of Tin2 are presented.

Inventors:
 [1];  [2]
  1. Berkeley, CA
  2. Albany, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
874537
Patent Number(s):
6409648
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
polynucleotides; encoding; trf1; binding; proteins; provides; novel; telomere; associated; protein; trf1-interacting; nuclear; tin2; hinders; specific; repeat; sequence; mediates; formation; tin2-trf1-telomeric; dna; complex; limits; telomerase; access; included; nucleic; acids; encode; mutants; methods; purifying; described; addition; drug; screening; assays; identify; drugs; mimic; andor; complement; effect; nucleic acid; binding protein; repeat sequence; /435/999/

Citation Formats

Campisi, Judith, and Kim, Sahn-Ho. Polynucleotides encoding TRF1 binding proteins. United States: N. p., 2002. Web.
Campisi, Judith, & Kim, Sahn-Ho. Polynucleotides encoding TRF1 binding proteins. United States.
Campisi, Judith, and Kim, Sahn-Ho. Tue . "Polynucleotides encoding TRF1 binding proteins". United States. https://www.osti.gov/servlets/purl/874537.
@article{osti_874537,
title = {Polynucleotides encoding TRF1 binding proteins},
author = {Campisi, Judith and Kim, Sahn-Ho},
abstractNote = {The present invention provides a novel telomere associated protein (Trf1-interacting nuclear protein 2 "Tin2") that hinders the binding of Trf1 to its specific telomere repeat sequence and mediates the formation of a Tin2-Trf1-telomeric DNA complex that limits telomerase access to the telomere. Also included are the corresponding nucleic acids that encode the Tin2 of the present invention, as well as mutants of Tin2. Methods of making, purifying and using Tin2 of the present invention are described. In addition, drug screening assays to identify drugs that mimic and/or complement the effect of Tin2 are presented.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Identification of a nonprocessive telomerase activity from mouse cells.
journal, February 1993


In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs
journal, March 1990


A mutant with a defect in telomere elongation leads to senescence in yeast
journal, May 1989


Antisense oligonucleotides for therapeutic intervention
journal, December 1991


Ribozymes and Their Medical Implications
journal, November 1988


Identification of a specific telomere terminal transferase activity in tetrahymena extracts
journal, December 1985


The telomere hypothesis of cellular aging
journal, July 1992


The serial cultivation of human diploid cell strains
journal, December 1961


Variability at the Telomeres of the Human X/Y Pseudoautosomal Region
journal, January 1986


Telomere length predicts replicative capacity of human fibroblasts.
journal, November 1992


The RNA component of human telomerase
journal, September 1995


Telomere loss and cancer
journal, March 1991


Telomerase RNAs of different ciliates have a common secondary structure and a permuted template.
journal, August 1994


Telomerases
journal, June 1992


Telomerase and Telomere-length Regulation: Lessons from Small Eukaryotes to Mammals
journal, January 1993


Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription
journal, November 1990


Strict relationship between dialyzed serum concentration and cellular life span in vitro
journal, October 1979


Specific association of human telomerase activity with immortal cells and cancer
journal, December 1994


TLC1: template RNA component of Saccharomyces cerevisiae telomerase
journal, October 1994


Telomeres shorten during ageing of human fibroblasts
journal, May 1990


Telomere telomere recombination provides an express pathway for telomere acquisition
journal, May 1990


A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis
journal, January 1989


Length of Telomeric Repeats in Neuroblastoma: Correlation with Prognosis and Other Biological Characteristics
journal, February 1992


Telomerase is processive.
journal, September 1991


A truncated human chromosome 16 associated with α thalassaemia is stabilized by addition of telomeric repeat (TTAGGG)n
journal, August 1990


Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.
journal, February 1994


Genetic disease detection and DNA amplification using cloned thermostable ligase.
journal, January 1991


The effects of nucleoside analogs on telomerase and telomeres in Tetrahymena
journal, January 1994


Telomeres, telomerase and senescence
journal, August 1990


Extensive telomere repeat arrays in mouse are hypervariable
journal, January 1990


Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989


Tying up the loose ends
journal, March 1990


Ribozymes and their medical implications
journal, November 1988


Developmentally programmed healing of chromosomes by telomerase in tetrahymena
journal, November 1991


Loss of Telomeric dna During Aging may Predispose Cells to Cancer (Review)
journal, October 1993


Telomerase activity in human ovarian carcinoma.
journal, April 1994


[5] Media and growth requirements
book, January 1979


The beginning of the ends
journal, January 1989


Chromosome first aid
journal, November 1991


Improved telomere detection using a telomere repeat probe (TTAGGG) n generated by PCR
journal, January 1991


Telomerase primer specificity and chromosome healing
journal, October 1991


Recognition and elongation of telomeres by telomerase
journal, January 1989


Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG) n
journal, January 1990


Functional evidence for an RNA template in telomerase
journal, February 1990


Immortal's Enzyme
journal, July 1994


Thein vivo application of ribozymes
journal, January 1990


Telomere loss: mitotic clock or genetic time bomb?
journal, March 1991


Replicative senescence: the human fibroblast comes of age
journal, September 1990


A conserved secondary structure for telomerase RNA
journal, October 1991


The Molecular Structure of Centromeres and Telomeres
journal, June 1984


Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma
journal, November 1992


Structure and function of telomeres
journal, April 1991


A theory of marginotomy
journal, September 1973


Inhibition of telomerase by G-quartet DMA structures
journal, April 1991