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Title: Methods and compositions for efficient nucleic acid sequencing

Abstract

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

Inventors:
 [1]
  1. 850 E. Greenwich Pl., Palo Alto, CA 94303
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
874493
Patent Number(s):
6401267
Assignee:
Drmanac; Radoje (850 E. Greenwich Pl., Palo Alto, CA 94303)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; compositions; efficient; nucleic; acid; sequencing; disclosed; novel; rapid; highly; based; hybridization; sets; oligonucleotide; probes; sequences; extremely; molecules; including; chromosomes; non-amplified; rna; sequenced; prior; cloning; subcloning; steps; solve; current; associated; technology; example; noise; signal; ratios; difficult; discrimination; attaching; fragments; surface; preparing; complex; labelling; species; nucleic acid; highly efficient; acid sequencing; oligonucleotide probe; /4/435/

Citation Formats

Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States: N. p., 2002. Web.
Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States.
Drmanac, Radoje. Tue . "Methods and compositions for efficient nucleic acid sequencing". United States. https://www.osti.gov/servlets/purl/874493.
@article{osti_874493,
title = {Methods and compositions for efficient nucleic acid sequencing},
author = {Drmanac, Radoje},
abstractNote = {Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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