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Title: Method and apparatus for synthesis of arrays of DNA probes

Abstract

The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing an image former that includes a light source that provides light to a micromirror device comprising an array of electronically addressable micromirrors, each of which can be selectively tilted between one of at least two positions. Projection optics receives the light reflected from the micromirrors along an optical axis and precisely images the micromirrors onto the active surface of the substrate, which may be used to activate the surface of the substrate. The first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different pattern of micromirrors, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto. The micromirror array can be controlled in conjunction with a DNA synthesizer supplying appropriate reagents to a flow cell containing the active substrate to control the sequencing of images presented by the micromirror array in coordination of the reagents provided tomore » the substrate.« less

Inventors:
 [1];  [1];  [1];  [1];  [1]
  1. Madison, WI
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
OSTI Identifier:
874365
Patent Number(s):
6375903
Application Number:
09/253,460
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
FG07-96ER13938; FG02-96ER45569
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; apparatus; synthesis; arrays; dna; probes; sequences; polypeptides; carried; patterning; process; active; surface; substrate; image; projected; utilizing; former; light; source; provides; micromirror; device; comprising; array; electronically; addressable; micromirrors; selectively; tilted; positions; projection; optics; receives; reflected; optical; axis; precisely; images; activate; level; bases; applied; followed; steps; subsequent; exposure; pattern; repeats; elements; dimensional; appropriate; base; bound; controlled; conjunction; synthesizer; supplying; reagents; flow; cell; containing; control; sequencing; coordination; provided; light source; substrate surface; light reflected; dna probe; /422/435/530/999/

Citation Formats

Cerrina, Francesco, Sussman, Michael R, Blattner, Frederick R, Singh-Gasson, Sangeet, and Green, Roland. Method and apparatus for synthesis of arrays of DNA probes. United States: N. p., 2002. Web.
Cerrina, Francesco, Sussman, Michael R, Blattner, Frederick R, Singh-Gasson, Sangeet, & Green, Roland. Method and apparatus for synthesis of arrays of DNA probes. United States.
Cerrina, Francesco, Sussman, Michael R, Blattner, Frederick R, Singh-Gasson, Sangeet, and Green, Roland. Tue . "Method and apparatus for synthesis of arrays of DNA probes". United States. https://www.osti.gov/servlets/purl/874365.
@article{osti_874365,
title = {Method and apparatus for synthesis of arrays of DNA probes},
author = {Cerrina, Francesco and Sussman, Michael R and Blattner, Frederick R and Singh-Gasson, Sangeet and Green, Roland},
abstractNote = {The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing an image former that includes a light source that provides light to a micromirror device comprising an array of electronically addressable micromirrors, each of which can be selectively tilted between one of at least two positions. Projection optics receives the light reflected from the micromirrors along an optical axis and precisely images the micromirrors onto the active surface of the substrate, which may be used to activate the surface of the substrate. The first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different pattern of micromirrors, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto. The micromirror array can be controlled in conjunction with a DNA synthesizer supplying appropriate reagents to a flow cell containing the active substrate to control the sequencing of images presented by the micromirror array in coordination of the reagents provided to the substrate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {4}
}

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Works referenced in this record:

Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists
journal, November 1996


Two-dimensional spatial light modulators: a tutorial
journal, May 1990


Light-generated oligonucleotide arrays for rapid DNA sequence analysis.
journal, May 1994


Excimer Laser Photolithography With 1:1 Wynne-Dyson Optics
conference, January 1988


Digital Light Processing and MEMS: reflecting the digital display needs of the networked society
conference, August 1996


The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
journal, June 1997

  • McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin
  • Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090
  • https://doi.org/10.1021/ja964427a

Broadband Deep-UV High NA Photolithography System
conference, July 1989


New Concepts in Projection Mask Aligners
journal, April 1975


Excimer laser projection lithography on a full-field scanning projection system
journal, May 1986