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Title: Multiflavor streptavidin

Abstract

Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin substitutes than for biotin.

Inventors:
 [1];  [2];  [3];  [1];  [4]
  1. Boston, MA
  2. Needham, MA
  3. Medfield, MA
  4. Del Mar, CA
Issue Date:
Research Org.:
Boston University
OSTI Identifier:
874334
Patent Number(s):
6368813
Assignee:
The Trustees of Boston University (Boston, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG02-93ER61656
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiflavor; streptavidin; compounds; methods; described; producing; mutants; changed; affinities; modifications; sequence; natural; create; amino; acid; substitutions; resulting; affinity; biotin; substitutes; amino acid; /435/530/999/

Citation Formats

Reznik, Gabriel O, Sano, Takeshi, Vajda, Sandor, Smith, Cassandra, and Cantor, Charles. Multiflavor streptavidin. United States: N. p., 2002. Web.
Reznik, Gabriel O, Sano, Takeshi, Vajda, Sandor, Smith, Cassandra, & Cantor, Charles. Multiflavor streptavidin. United States.
Reznik, Gabriel O, Sano, Takeshi, Vajda, Sandor, Smith, Cassandra, and Cantor, Charles. Tue . "Multiflavor streptavidin". United States. https://www.osti.gov/servlets/purl/874334.
@article{osti_874334,
title = {Multiflavor streptavidin},
author = {Reznik, Gabriel O and Sano, Takeshi and Vajda, Sandor and Smith, Cassandra and Cantor, Charles},
abstractNote = {Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin substitutes than for biotin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

Influence of endogenous biotin on the biodistribution of labelled biotin derivatives in mice
journal, January 1995


Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120.
journal, February 1995


Molecular Origins of the Slow Streptavidin-Biotin Dissociation Kinetics
journal, November 1995


Intermolecular forces and energies between ligands and receptors
journal, October 1994