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Title: Invasive cleavage of nucleic acids

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Inventors:
 [1];  [1];  [1];  [1];  [1]
  1. (Madison, WI)
Issue Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI (United States)
OSTI Identifier:
874241
Patent Number(s):
6348314
Assignee:
Third Wave Technologies, Inc. (Madison, WI) CHO
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
invasive; cleavage; nucleic; acids; relates; means; detection; characterization; acid; sequences; variations; methods; forming; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; nucleic acid; acid sequence; specific nucleic; /435/

Citation Formats

Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann D., and Dahlberg, James E. Invasive cleavage of nucleic acids. United States: N. p., 2002. Web.
Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann D., & Dahlberg, James E. Invasive cleavage of nucleic acids. United States.
Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann D., and Dahlberg, James E. Tue . "Invasive cleavage of nucleic acids". United States. https://www.osti.gov/servlets/purl/874241.
@article{osti_874241,
title = {Invasive cleavage of nucleic acids},
author = {Prudent, James R. and Hall, Jeff G. and Lyamichev, Victor I. and Brow, Mary Ann D. and Dahlberg, James E.},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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