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Title: Apparatus and method for collection and concentration of respirable particles into a small fluid volume

Abstract

An apparatus and method for the collection of respirable particles and concentration of such particles into a small fluid volume. The apparatus captures and concentrates small (1-10 .mu.m) respirable particles into a sub-millileter volume of fluid. The method involves a two step operation, collection and concentration: wherein collection of particles is by a wetted surface having small vertical slits that act as capillary channels; and concentration is carried out by transfer of the collected particles to a small volume (sub-milliliter) container by centrifugal force whereby the particles are forced through the vertical slits and contact a non-wetted wall surface, and are deflected to the bottom where they are contained for analysis, such as a portable flow cytometer or a portable PCR DNA analysis system.

Inventors:
 [1];  [2]
  1. San Leandro, CA
  2. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874197
Patent Number(s):
6337213
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; method; collection; concentration; respirable; particles; fluid; volume; captures; concentrates; 1-10; mum; sub-millileter; involves; step; operation; wetted; surface; vertical; slits; capillary; channels; carried; transfer; collected; sub-milliliter; container; centrifugal; force; forced; contact; non-wetted; wall; deflected; bottom; contained; analysis; portable; flow; cytometer; pcr; dna; centrifugal force; fluid volume; /436/55/422/

Citation Formats

Simon, Jonathan N, and Brown, Steve B. Apparatus and method for collection and concentration of respirable particles into a small fluid volume. United States: N. p., 2002. Web.
Simon, Jonathan N, & Brown, Steve B. Apparatus and method for collection and concentration of respirable particles into a small fluid volume. United States.
Simon, Jonathan N, and Brown, Steve B. Tue . "Apparatus and method for collection and concentration of respirable particles into a small fluid volume". United States. https://www.osti.gov/servlets/purl/874197.
@article{osti_874197,
title = {Apparatus and method for collection and concentration of respirable particles into a small fluid volume},
author = {Simon, Jonathan N and Brown, Steve B},
abstractNote = {An apparatus and method for the collection of respirable particles and concentration of such particles into a small fluid volume. The apparatus captures and concentrates small (1-10 .mu.m) respirable particles into a sub-millileter volume of fluid. The method involves a two step operation, collection and concentration: wherein collection of particles is by a wetted surface having small vertical slits that act as capillary channels; and concentration is carried out by transfer of the collected particles to a small volume (sub-milliliter) container by centrifugal force whereby the particles are forced through the vertical slits and contact a non-wetted wall surface, and are deflected to the bottom where they are contained for analysis, such as a portable flow cytometer or a portable PCR DNA analysis system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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