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Title: Modified electrokinetic sample injection method in chromatography and electrophoresis analysis

Abstract

A sample injection method for horizontal configured multiple chromatography or electrophoresis units, each containing a number of separation/analysis channels, that enables efficient introduction of analyte samples. This method for loading when taken in conjunction with horizontal microchannels allows much reduced sample volumes and a means of sample stacking to greatly reduce the concentration of the sample. This reduction in the amount of sample can lead to great cost savings in sample preparation, particularly in massively parallel applications such as DNA sequencing. The essence of this method is in preparation of the input of the separation channel, the physical sample introduction, and subsequent removal of excess material. By this method, sample volumes of 100 nanoliter to 2 microliters have been used successfully, compared to the typical 5 microliters of sample required by the prior separation/analysis method.

Inventors:
 [1];  [1]
  1. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874127
Patent Number(s):
6319379
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
modified; electrokinetic; sample; injection; method; chromatography; electrophoresis; analysis; horizontal; configured; multiple; units; containing; separationanalysis; channels; enables; efficient; introduction; analyte; samples; loading; conjunction; microchannels; allows; reduced; volumes; means; stacking; greatly; reduce; concentration; reduction; amount; lead; cost; savings; preparation; massively; parallel; applications; dna; sequencing; essence; input; separation; channel; physical; subsequent; removal; excess; material; 100; nanoliter; microliters; successfully; compared; typical; required; prior; analysis method; /204/

Citation Formats

Davidson, J Courtney, and Balch, Joseph W. Modified electrokinetic sample injection method in chromatography and electrophoresis analysis. United States: N. p., 2001. Web.
Davidson, J Courtney, & Balch, Joseph W. Modified electrokinetic sample injection method in chromatography and electrophoresis analysis. United States.
Davidson, J Courtney, and Balch, Joseph W. Mon . "Modified electrokinetic sample injection method in chromatography and electrophoresis analysis". United States. https://www.osti.gov/servlets/purl/874127.
@article{osti_874127,
title = {Modified electrokinetic sample injection method in chromatography and electrophoresis analysis},
author = {Davidson, J Courtney and Balch, Joseph W},
abstractNote = {A sample injection method for horizontal configured multiple chromatography or electrophoresis units, each containing a number of separation/analysis channels, that enables efficient introduction of analyte samples. This method for loading when taken in conjunction with horizontal microchannels allows much reduced sample volumes and a means of sample stacking to greatly reduce the concentration of the sample. This reduction in the amount of sample can lead to great cost savings in sample preparation, particularly in massively parallel applications such as DNA sequencing. The essence of this method is in preparation of the input of the separation channel, the physical sample introduction, and subsequent removal of excess material. By this method, sample volumes of 100 nanoliter to 2 microliters have been used successfully, compared to the typical 5 microliters of sample required by the prior separation/analysis method.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}