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Title: Agouti polypeptide compositions

Abstract

Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.

Inventors:
 [1];  [2];  [3]
  1. Orinda, CA
  2. Lakewood, OH
  3. Kingston, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
OSTI Identifier:
874092
Patent Number(s):
6310034
Application Number:
09/034,088
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
agouti; polypeptide; compositions; disclosed; methods; comprising; novel; polypeptides; polynucleotides; encode; dna; segments; encoding; proteins; derived; human; murine; cell; lines; variety; diagnostic; therapeutic; applications; kits; devices; provided; identifying; compounds; inhibitors; activity; altering; fatty; acid; synthetase; intracellular; calcium; levels; transformed; cells; fatty acid; cell line; /514/530/

Citation Formats

Woychik, Richard P, Bultman, Scott J, and Michaud, Edward J. Agouti polypeptide compositions. United States: N. p., 2001. Web.
Woychik, Richard P, Bultman, Scott J, & Michaud, Edward J. Agouti polypeptide compositions. United States.
Woychik, Richard P, Bultman, Scott J, and Michaud, Edward J. Tue . "Agouti polypeptide compositions". United States. https://www.osti.gov/servlets/purl/874092.
@article{osti_874092,
title = {Agouti polypeptide compositions},
author = {Woychik, Richard P and Bultman, Scott J and Michaud, Edward J},
abstractNote = {Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {10}
}

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Works referenced in this record:

Fatty acid synthase and adipsin mRNA levels in obese and lean JCR:LA-cp rats: effect of diet.
journal, January 1992


An inherited limb deformity created by insertional mutagenesis in a transgenic mouse
journal, November 1985


A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation.
journal, March 1994


Molecular structure and chromosomal mapping of the human homolog of the agouti gene.
journal, October 1994


Genomic Organization and Molecular Genetics of the Agouti Locus in the Mousea
journal, December 1991


Mechanisms for the pleiotropic effects of the agouti gene.
journal, May 1995


Combined effects of insulin treatment and adipose tissue-specific agouti expression on the development of obesity
journal, February 1997


Structure and function of ASP , the human homolog of the mouse agouti gene
journal, January 1995


When does homology mean something else?
journal, September 1987


Role of melanocortinergic neurons in feeding and the agouti obesity syndrome
journal, January 1997


Overexpression of anAgouticDNA in the Skin of Transgenic Mice Recapitulates Dominant Coat Color Phenotypes of Spontaneous Mutants
journal, January 1996


Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-IIIA3
journal, August 1973


Molecular characterization of the mouse agouti locus
journal, December 1992


“Homology” in proteins and nucleic acids: A terminology muddle and a way out of it
journal, August 1987


A transgenic mouse assay for agouti protein activity.
journal, May 1995


Myosin light chain enhancer activates muscle-specific, developmentally regulated gene expression in transgenic mice.
journal, October 1989


Molecular and Developmental Genetics of Mouse Coat Color
journal, December 1994


Targeted Disruption of the Melanocortin-4 Receptor Results in Obesity in Mice
journal, January 1997


The effects of calcium channel blockade on agouti ‐induced obesity
journal, December 1996


Agouti structure and function: characterization of a potent .alpha.-melanocyte stimulating hormone receptor antagonist
journal, September 1995


Phaeomelanin synthesis and obesity in mice
journal, September 1978


Molecular Basis of the Pleiotropic Phenotype of Mice Carrying the hypervariable yellow (Ahvy) Mutation at the agouti Locus
journal, February 1996


Coupled Site-Directed Mutagenesis/Transgenesis Identifies Important Functional Domains of the Mouse Agouti Protein
journal, September 1996


Molecular and genetic characterization of a radiation-induced structural rearrangement in mouse chromosome 2 causing mutations at the limb deformity and agouti loci.
journal, April 1990


Agouti regulation of intracellular calcium: role of melanocortin receptors
journal, March 1997


Upregulation of adipocyte metabolism by agouti protein: possible paracrine actions in yellow mouse obesity
journal, January 1996


Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus.
journal, May 1995


Increase in cytosolic free Ca2+ in corticotropin-stimulated white adipocytes
journal, March 1994


Adrenalectomy and response to corticosterone and MSH in the genetically obese yellow mouse
journal, February 1989


Pancreatic Islet Cells in Preobese Yellow Avy/- Mice: Relation to Adult Hyperinsulinemia and Obesity
journal, June 1994


Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989


Positional cloning of the mouse obese gene and its human homologue
journal, December 1994


Mutations in the Carboxyl Terminus of the Agouti Protein Decrease Agouti Inhibition of Ligand Binding to the Melanocortin Receptors
journal, February 1997


Molecular characterization of a region of DNA associated with mutations at the agouti locus in the mouse.
journal, September 1991


Genetic Organization of the agouti Region of the Mouse
journal, September 1987


The Role of the agouti Gene in the Yellow Obese Syndrome
journal, September 1997


Molecular cloning of a novel melanocortin receptor.
journal, April 1993


Agouti protein is an antagonist of the melanocyte-stimulating-hormone receptor
journal, October 1994


Role of the agouti gene in obesity
journal, November 1997


Obesity, diabetes, and neoplasia in yellow A vy /‐ mice: ectopic expression of the agouti gene
journal, May 1994


Agouti regulation of intracellular calcium: role in the insulin resistance of viable yellow mice.
journal, May 1995


Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone.
journal, August 1989


Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur.
journal, May 1995


Molecular Cloning, Expression, and Characterization of a Fifth Melanocortin Receptor
journal, May 1994


Allelic Variation Within the Emv-15 Locus Defines Genomic Sequences Closely Linked to the agouti Locus on Mouse Chromosome 2
journal, September 1987


Impaired Recovery of Vascular Smooth Muscle Intracellular Calcium Following Agonist Stimulation in Insulin Resistant (Zucker Obese) Rats
journal, June 1993